Sponge-Like: A New Protocol for Preparing Bacterial Ghosts
Joint Authors
Amara, Amro A.
Salem-Bekhit, Mounir M.
Alanazi, Fars K.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Bacterial Ghosts (BGs) received an increasing interest in the recent years for their promising medicinal and pharmaceutical applications.
In this study, for the first time we introduce a new protocol for BGs production.
E.
coli BL21 (DE3) pLysS (Promega) was used as a model to establish a general protocol for BGs preparation.
The protocol is based on using active chemical compounds in concentrations less than the Minimum Inhibition Concentration (MIC).
Those chemical compounds are SDS, NaOH, and H2O2.
Plackett-Burman experimental design was used to map the best conditions for BGs production.
Normal and electronic microscopes were used to evaluate the BGs quality (BGQ).
Spectrophotometer was used to evaluate the amount of the released protein and DNA.
Agarose gel electrophoresis was used to determine the existence of any residue of DNA after each BGs preparation.
Viable cells, which existed after running this protocol, were subjected to lysis by inducing the lysozyme gene carried on pLysS plasmid.
This protocol is able to produce BGs that can be used in different biotechnological applications.
American Psychological Association (APA)
Amara, Amro A.& Salem-Bekhit, Mounir M.& Alanazi, Fars K.. 2013. Sponge-Like: A New Protocol for Preparing Bacterial Ghosts. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1033049
Modern Language Association (MLA)
Amara, Amro A.…[et al.]. Sponge-Like: A New Protocol for Preparing Bacterial Ghosts. The Scientific World Journal No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1033049
American Medical Association (AMA)
Amara, Amro A.& Salem-Bekhit, Mounir M.& Alanazi, Fars K.. Sponge-Like: A New Protocol for Preparing Bacterial Ghosts. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1033049
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033049