Cytokine Response of Cultured Skeletal Muscle Cells Stimulated with Proinflammatory Factors Depends on Differentiation Stage
Joint Authors
Podbregar, Matej
Lainscak, Mitja
Prelovsek, Oja
Mars, Tomaz
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Myoblast proliferation and myotube formation are critical early events in skeletal muscle regeneration.
The attending inflammation and cytokine signaling are involved in regulation of skeletal muscle cell proliferation and differentiation.
Secretion of muscle-derived cytokines upon exposure to inflammatory factors may depend on the differentiation stage of regenerating muscle cells.
Cultured human myoblasts and myotubes were exposed to 24-hour treatment with tumor necrosis factor (TNF)-α or lipopolysaccharide (LPS).
Secretion of interleukin 6 (IL-6), a major muscle-derived cytokine, and interleukin 1 (IL-1), an important regulator of inflammatory response, was measured 24 hours after termination of TNF-α or LPS treatment.
Myoblasts pretreated with TNF-α or LPS displayed robustly increased IL-6 secretion during the 24-hour period after removal of treatments, while IL-1 secretion remained unaltered.
IL-6 secretion was also increased in myotubes, but the response was less pronounced compared with myoblasts.
In contrast to myoblasts, IL-1 secretion was markedly stimulated in LPS-pretreated myotubes.
We demonstrate that preceding exposure to inflammatory factors stimulates a prolonged upregulation of muscle-derived IL-6 and/or IL-1 in cultured skeletal muscle cells.
Our findings also indicate that cytokine response to inflammatory factors in regenerating skeletal muscle partially depends on the differentiation stage of myogenic cells.
American Psychological Association (APA)
Podbregar, Matej& Lainscak, Mitja& Prelovsek, Oja& Mars, Tomaz. 2013. Cytokine Response of Cultured Skeletal Muscle Cells Stimulated with Proinflammatory Factors Depends on Differentiation Stage. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1033118
Modern Language Association (MLA)
Podbregar, Matej…[et al.]. Cytokine Response of Cultured Skeletal Muscle Cells Stimulated with Proinflammatory Factors Depends on Differentiation Stage. The Scientific World Journal No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1033118
American Medical Association (AMA)
Podbregar, Matej& Lainscak, Mitja& Prelovsek, Oja& Mars, Tomaz. Cytokine Response of Cultured Skeletal Muscle Cells Stimulated with Proinflammatory Factors Depends on Differentiation Stage. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1033118
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033118