Development of a pyrG Mutant of Aspergillus oryzae Strain S1 as a Host for the Production of Heterologous Proteins
Joint Authors
Abdul Murad, Abdul Munir
Ling, Selina Oh Siew
Storms, Reginald
Zheng, Yun
Rodzi, Mohd Rohaizad Mohd
Illias, Rosli Md
Abu Bakar, Farah Diba
Mahadi, Nor Muhammad
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The ease with which auxotrophic strains and genes that complement them can be manipulated, as well as the stability of auxotrophic selection systems, are amongst the advantages of using auxotrophic markers to produce heterologous proteins.
Most auxotrophic markers in Aspergillus oryzae originate from chemical or physical mutagenesis that may yield undesirable mutations along with the mutation of interest.
An auxotrophic A.
oryzae strain S1 was generated by deleting the orotidine-5′-monophosphate decarboxylase gene (pyrG) by targeted gene replacement.
The uridine requirement of the resulting strain GR6 pyrGΔ0 was complemented by plasmids carrying a pyrG gene from either Aspergillus nidulans or A.
oryzae.
β-Galactosidase expression by strain GR6 pyrGΔ0 transformed with an A.
niger plasmid encoding a heterologous β-galactosidase was at least 150 times more than that obtained with the untransformed strain.
Targeted gene replacement is thus an efficient way of developing auxotrophic mutants in A.
oryzae and the auxotrophic strain GR6 pyrGΔ0 facilitated the production of a heterologous protein in this fungus.
American Psychological Association (APA)
Ling, Selina Oh Siew& Storms, Reginald& Zheng, Yun& Rodzi, Mohd Rohaizad Mohd& Mahadi, Nor Muhammad& Illias, Rosli Md…[et al.]. 2013. Development of a pyrG Mutant of Aspergillus oryzae Strain S1 as a Host for the Production of Heterologous Proteins. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1033152
Modern Language Association (MLA)
Ling, Selina Oh Siew…[et al.]. Development of a pyrG Mutant of Aspergillus oryzae Strain S1 as a Host for the Production of Heterologous Proteins. The Scientific World Journal No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1033152
American Medical Association (AMA)
Ling, Selina Oh Siew& Storms, Reginald& Zheng, Yun& Rodzi, Mohd Rohaizad Mohd& Mahadi, Nor Muhammad& Illias, Rosli Md…[et al.]. Development of a pyrG Mutant of Aspergillus oryzae Strain S1 as a Host for the Production of Heterologous Proteins. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1033152
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033152