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Preamplification Procedure for the Analysis of Ancient DNA Samples
Joint Authors
Del Gaudio, S.
Di Bernardo, Giovanni
Galderisi, Umberto
Thanassoulas, Theodoros
Pitsios, Theodoros
Cipollaro, Marilena
Cirillo, Alessandra
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
In ancient DNA studies the low amount of endogenous DNA represents a limiting factor that often hampers the result achievement.
In this study we extracted the DNA from nine human skeletal remains of different ages found in the Byzantine cemetery of Abdera Halkidiki and in the medieval cemetery of St.
Spiridion in Rhodes (Greece).
Real-time quantitative polymerase chain reaction (qPCR) was used to detect in the extracts the presence of PCR inhibitors and to estimate the DNA content.
As mitochondrial DNA was detected in all samples, amplification of nuclear targets, as amelogenin and the polymorphism M470V of the transmembrane conductance regulator gene, yielded positive results in one case only.
In an effort to improve amplification success, we applied, for the first time in ancient DNA, a preamplification strategy based on TaqMan PreAmp Master Mix.
A comparison between results obtained from nonpreamplified and preamplified samples is reported.
Our data, even if preliminary, show that the TaqMan PreAmp procedure may improve the sensitivity of qPCR analysis.
American Psychological Association (APA)
Del Gaudio, S.& Cirillo, Alessandra& Di Bernardo, Giovanni& Galderisi, Umberto& Thanassoulas, Theodoros& Pitsios, Theodoros…[et al.]. 2013. Preamplification Procedure for the Analysis of Ancient DNA Samples. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1033252
Modern Language Association (MLA)
Del Gaudio, S.…[et al.]. Preamplification Procedure for the Analysis of Ancient DNA Samples. The Scientific World Journal No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1033252
American Medical Association (AMA)
Del Gaudio, S.& Cirillo, Alessandra& Di Bernardo, Giovanni& Galderisi, Umberto& Thanassoulas, Theodoros& Pitsios, Theodoros…[et al.]. Preamplification Procedure for the Analysis of Ancient DNA Samples. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1033252
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033252