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The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil
Joint Authors
Hofer, Ernesto
Mendes-Marques, Carina Lucena
Silveira Filho, Vladimir da Mota
da Costa, Ana Paula Rocha
Nunes, Mariana de Lira
Silva Filho, Sandoval Vieira da
Figueirôa, Ângela Cristina Torres de Araújo
Leal, Nilma Cintra
de Almeida, Alzira Maria Paiva
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-25
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic.
After a quiescent period, 4 rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single-tube nested PCR (MSTNPCR); 2 of these were also ctxA (cholera toxin) positive.
From May to June 2012, 30 V.
cholerae O1 isolates were obtained by culturing samples.
These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S-23S PCR (ISR-PCR), and pulsed field gel electrophoresis (PFGE).
The isolates were positive for the rfbN gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE.
Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemic V.
cholerae O1 in the region.
American Psychological Association (APA)
da Costa, Ana Paula Rocha& Nunes, Mariana de Lira& Silva Filho, Sandoval Vieira da& Figueirôa, Ângela Cristina Torres de Araújo& Hofer, Ernesto& de Almeida, Alzira Maria Paiva…[et al.]. 2013. The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1033263
Modern Language Association (MLA)
da Costa, Ana Paula Rocha…[et al.]. The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil. The Scientific World Journal No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1033263
American Medical Association (AMA)
da Costa, Ana Paula Rocha& Nunes, Mariana de Lira& Silva Filho, Sandoval Vieira da& Figueirôa, Ângela Cristina Torres de Araújo& Hofer, Ernesto& de Almeida, Alzira Maria Paiva…[et al.]. The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1033263
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033263