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Dengue Virus Type 2: Protein Binding and Active Replication in Human Central Nervous System Cells
Joint Authors
Salazar, Ma. Isabel
Pérez-García, Marissa
Terreros-Tinoco, Marisol
Diegopérez-Ramírez, Jaime
Ramírez-Reyes, Alma Griselda
Aguilera, Penélope
Cedillo-Barrón, Leticia
García-Flores, María Martha
Castro-Mussot, María Eugenia
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
An increased number of dengue cases with neurological complications have been reported in recent years.
The lack of reliable animal models for dengue has hindered studies on dengue virus (DENV) pathogenesis and cellular tropism in vivo.
We further investigate the tropism of DENV for the human central nervous system (CNS), characterizing DENV interactions with cell surface proteins in human CNS cells by virus overlay protein binding assays (VOPBA) and coimmunoprecipitations.
In VOPBA, three membrane proteins (60, 70, and 130 kDa) from the gray matter bound the entire virus particle, whereas only a 70 kDa protein bound in white matter.
The coimmunoprecipitation assays revealed three proteins from gray matter consistently binding virus particles, one clearly distinguishable protein (~32 kDa) and two less apparent proteins (100 and 130 kDa).
Monoclonal anti-NS3 targeted the virus protein in primary cell cultures of human CNS treated with DENV-2, which also stained positive for NeuH, a neuron-specific marker.
Thus, our results indicate (1) that DENV-2 exhibited a direct tropism for human neurons and (2) that human neurons sustain an active DENV replication as was demonstrated by the presence of the NS3 viral antigen in primary cultures of these cells treated with DENV-2.
American Psychological Association (APA)
Salazar, Ma. Isabel& Pérez-García, Marissa& Terreros-Tinoco, Marisol& Castro-Mussot, María Eugenia& Diegopérez-Ramírez, Jaime& Ramírez-Reyes, Alma Griselda…[et al.]. 2013. Dengue Virus Type 2: Protein Binding and Active Replication in Human Central Nervous System Cells. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1033420
Modern Language Association (MLA)
Salazar, Ma. Isabel…[et al.]. Dengue Virus Type 2: Protein Binding and Active Replication in Human Central Nervous System Cells. The Scientific World Journal No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1033420
American Medical Association (AMA)
Salazar, Ma. Isabel& Pérez-García, Marissa& Terreros-Tinoco, Marisol& Castro-Mussot, María Eugenia& Diegopérez-Ramírez, Jaime& Ramírez-Reyes, Alma Griselda…[et al.]. Dengue Virus Type 2: Protein Binding and Active Replication in Human Central Nervous System Cells. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1033420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033420