![](/images/graphics-bg.png)
Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube
Joint Authors
Lee, So-Ra
Lee, Hyo-Eun
Kang, Yun Ok
Hwang, Wan-Seok
Choi, Seong-Ho
Source
Advances in Materials Science and Engineering
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-13
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Bienzymatic acetylcholinesterase (AChE) and choline oxidase (ChOx) immobilized biosensor based on a phenyl carboxylic acid-grafted multiwalled carbon nanotube (MWNT) modified glass carbon electrode (GCE) and carbon-screen printed electrode (SPE) was fabricated for acetylcholine detection in human blood samples.
Phenyl carboxylic acid-modified MWNT supports were prepared by electrochemical polymerization of 4-carboxyphenyl diazonium salts, which were synthesized by an amine group and sodium nitrite, on the surface of the MWNT-modified GCE and SPE in 0.1 M PBS.
The successful fabrication of the AChE-ChOx-immobilized biosensor was confirmed via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV).
The sensing range of the biosensor based on a GCE and SPE was 1.0~10 μM and 10~100 μM, respectively.
The interfering effect of 0.1 M L-ascorbic acid, 0.1 M L-cysteine, and 0.1 M uric acid to 0.1 M acetylcholine was 3.00%, 9.00%, and 3.00%, respectively.
Acetylcholine in a human blood sample was detected by the AChE-ChOx-immobilized biosensor.
American Psychological Association (APA)
Lee, So-Ra& Lee, Hyo-Eun& Kang, Yun Ok& Hwang, Wan-Seok& Choi, Seong-Ho. 2014. Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube. Advances in Materials Science and Engineering،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1034333
Modern Language Association (MLA)
Lee, So-Ra…[et al.]. Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube. Advances in Materials Science and Engineering No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1034333
American Medical Association (AMA)
Lee, So-Ra& Lee, Hyo-Eun& Kang, Yun Ok& Hwang, Wan-Seok& Choi, Seong-Ho. Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube. Advances in Materials Science and Engineering. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1034333
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034333