Characterisation of Potential Antidiabetic-Related Proteins from Pleurotus pulmonarius (Fr.) Quél. (Grey Oyster Mushroom) by MALDI-TOFTOF Mass Spectrometry
Joint Authors
Abdullah, Noorlidah
Wahab, Nurul Azwa Abd.
Aminudin, Norhaniza
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Pleurotus pulmonarius has been reported to have a potent remedial effect on diabetic property and considered to be an alternative for type 2 diabetes mellitus treatment.
This study aimed to investigate the antidiabetic properties of ammonium sulphate precipitated protein fractions from P.
pulmonarius basidiocarps.
Preliminary results demonstrated that 30% (NH4)2SO4 precipitated fraction (F30) inhibited Saccharomyces cerevisiae α-glucosidase activity (24.18%), and 100% (NH4)2SO4 precipitated fraction (F100) inhibited porcine pancreatic α-amylase activity (41.80%).
Following RP-HPLC purification, peak 3 from F30 fraction demonstrated inhibition towards α-glucosidase at the same time with meagre inhibition towards α-amylase activity.
Characterisation of proteins using MALDI-TOF/TOF MS demonstrated the presence of four different proteins, which could be implicated in the regulation of blood glucose level via various mechanisms.
Therefore, this study revealed the presence of four antidiabetic-related proteins which are profilin-like protein, glyceraldehyde-3-phosphate dehydrogenase-like protein, trehalose phosphorylase-like (TP-like) protein, and catalase-like protein.
Hence, P.
pulmonarius basidiocarps have high potential in lowering blood glucose level, reducing insulin resistance and vascular complications.
American Psychological Association (APA)
Wahab, Nurul Azwa Abd.& Abdullah, Noorlidah& Aminudin, Norhaniza. 2014. Characterisation of Potential Antidiabetic-Related Proteins from Pleurotus pulmonarius (Fr.) Quél. (Grey Oyster Mushroom) by MALDI-TOFTOF Mass Spectrometry. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034381
Modern Language Association (MLA)
Wahab, Nurul Azwa Abd.…[et al.]. Characterisation of Potential Antidiabetic-Related Proteins from Pleurotus pulmonarius (Fr.) Quél. (Grey Oyster Mushroom) by MALDI-TOFTOF Mass Spectrometry. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1034381
American Medical Association (AMA)
Wahab, Nurul Azwa Abd.& Abdullah, Noorlidah& Aminudin, Norhaniza. Characterisation of Potential Antidiabetic-Related Proteins from Pleurotus pulmonarius (Fr.) Quél. (Grey Oyster Mushroom) by MALDI-TOFTOF Mass Spectrometry. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034381