Downregulation of MDR1 Gene by Cepharanthine Hydrochloride Is Related to the Activation of c-JunJNK in K562ADR Cells
Joint Authors
Ning, Wang
Wang, Yafeng
Guo, Xiaojuan
Zhou, Yubing
Zhang, Jingmin
Jiang, Jinhua
Ma, Fang
Wang, Qingduan
Li, Han
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The purpose of the study was to determine the signal transduction mechanism of cepharanthine hydrochloride (CH) on reversing tumor multidrug resistance.
RT-PCR and Western blot analysis were used to determine the effects of CH on the expression of MDR1 mRNA and P-glycoprotein in K562/ADR cells when CH was used alone and combined with SP600125, a JNK inhibitor, to explore the effects of CH on JNK pathway.
Western blot analysis was used to determine the effects of CH on c-Jun protein expression and phosphorylation, to explore the regulating effects of CH on c-Jun and phosphorylated c-Jun (p-c-Jun) proteins.
Our results showed that the inhibitory effect of CH on MDR1 mRNA increased with the concentrations of CH (5.0, 10.0, and 20.0 μM) and the inhibitory effects of CH on MDR1 mRNA and P-glycoprotein increased with the incubation time of CH (0, 12, 24, 36, and 48 hours).
The inhibitory effect was weakened after CH combined with SP600125.
The expressions of c-Jun and p-c-Jun proteins increased with the incubation time of CH (0, 6, 12, and 24 hours).
These findings suggest that CH downregulated the expressions of MDR1 mRNA and P-glycoprotein in a time and concentration manner; the mechanism may be mediated via activating c-Jun/JNK pathway.
American Psychological Association (APA)
Li, Han& Wang, Yafeng& Guo, Xiaojuan& Zhou, Yubing& Zhang, Jingmin& Ning, Wang…[et al.]. 2014. Downregulation of MDR1 Gene by Cepharanthine Hydrochloride Is Related to the Activation of c-JunJNK in K562ADR Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1034390
Modern Language Association (MLA)
Li, Han…[et al.]. Downregulation of MDR1 Gene by Cepharanthine Hydrochloride Is Related to the Activation of c-JunJNK in K562ADR Cells. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1034390
American Medical Association (AMA)
Li, Han& Wang, Yafeng& Guo, Xiaojuan& Zhou, Yubing& Zhang, Jingmin& Ning, Wang…[et al.]. Downregulation of MDR1 Gene by Cepharanthine Hydrochloride Is Related to the Activation of c-JunJNK in K562ADR Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1034390
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034390