Proteomics Analysis for Finding Serum Markers of Ovarian Cancer

Joint Authors

Cheng, Yushan
Zhang, Nawei
Wang, Shengdian
Zhang, Zhenyu
Liu, Chongdong

Source

BioMed Research International

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-08-31

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Medicine

Abstract EN

A combination of peptide ligand library beads (PLLB) and 1D gel liquid chromatography-mass spectrometry/mass spectrometry (1DGel-LC-MS/MS) was employed to analyze serum samples from patients with ovarian cancer and from healthy controls.

Proteomic analysis identified 1200 serum proteins, among which 57 proteins were upregulated and 10 were downregulated in the sera from cancer patients.

Retinol binding protein 4 (RBP4) is highly upregulated in the ovarian cancer serum samples.

ELISA was employed to measure plasma concentrations of RBP4 in 80 samples from ovarian cancer patients, healthy individuals, myoma patients, and patients with benign ovarian tumor, respectively.

The plasma concentrations of RBP4 ranging from 76.91 to 120.08 ng/mL with the mean value 89.13 ± 1.67 ng/mL in ovarian cancer patients are significantly higher than those in healthy individuals ( 10.85 ± 2.38 ng/mL).

Results were further confirmed with immunohistochemistry, demonstrating that RBP4 expression levels in normal ovarian tissue were lower than those in ovarian cancer tissues.

Our results suggested that RBP4 is a potential biomarker for diagnostic of screening ovarian cancer.

American Psychological Association (APA)

Cheng, Yushan& Liu, Chongdong& Zhang, Nawei& Wang, Shengdian& Zhang, Zhenyu. 2014. Proteomics Analysis for Finding Serum Markers of Ovarian Cancer. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034395

Modern Language Association (MLA)

Cheng, Yushan…[et al.]. Proteomics Analysis for Finding Serum Markers of Ovarian Cancer. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1034395

American Medical Association (AMA)

Cheng, Yushan& Liu, Chongdong& Zhang, Nawei& Wang, Shengdian& Zhang, Zhenyu. Proteomics Analysis for Finding Serum Markers of Ovarian Cancer. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034395

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1034395