Expression of Phosphocitrate-Targeted Genes in Osteoarthritis Menisci
Joint Authors
Hanley, Edward N.
Mauerhan, David R.
Steuerwald, Nury M.
Ingram, Jane
Kneisl, Jeffrey S.
Sun, Yubo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-23
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Phosphocitrate (PC) inhibited calcium crystal-associated osteoarthritis (OA) in Hartley guinea pigs.
However, the molecular mechanisms remain elusive.
This study sought to determine PC targeted genes and the expression of select PC targeted genes in OA menisci to test hypothesis that PC exerts its disease modifying activity in part by reversing abnormal expressions of genes involved in OA.
We found that PC downregulated the expression of numerous genes classified in immune response, inflammatory response, and angiogenesis, including chemokine (C-C motif) ligand 5, Fc fragment of IgG, low affinity IIIb receptor (FCGR3B), and leukocyte immunoglobulin-like receptor, subfamily B member 3 (LILRB3).
In contrast, PC upregulated the expression of many genes classified in skeletal development, including collagen type II alpha1, fibroblast growth factor receptor 3 (FGFR3), and SRY- (sex determining region Y-) box 9 (SOX-9).
Immunohistochemical examinations revealed higher levels of FCGR3B and LILRB3 and lower level of SOX-9 in OA menisci.
These findings indicate that OA is a disease associated with immune system activation and decreased expression of SOX-9 gene in OA menisci.
PC exerts its disease modifying activity on OA, at least in part, by targeting immune system activation and the production of extracellular matrix and selecting chondroprotective proteins.
American Psychological Association (APA)
Sun, Yubo& Mauerhan, David R.& Steuerwald, Nury M.& Ingram, Jane& Kneisl, Jeffrey S.& Hanley, Edward N.. 2014. Expression of Phosphocitrate-Targeted Genes in Osteoarthritis Menisci. BioMed Research International،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-1034400
Modern Language Association (MLA)
Sun, Yubo…[et al.]. Expression of Phosphocitrate-Targeted Genes in Osteoarthritis Menisci. BioMed Research International No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-1034400
American Medical Association (AMA)
Sun, Yubo& Mauerhan, David R.& Steuerwald, Nury M.& Ingram, Jane& Kneisl, Jeffrey S.& Hanley, Edward N.. Expression of Phosphocitrate-Targeted Genes in Osteoarthritis Menisci. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-1034400
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034400