Evaluation of protective Immunity of 28kd-gst as a vaccineagainst Expermental schistorsoma mansoni

Abstract EN

Schistosoma mansion GST was purified from adult worm homogenates by affinity chromatography.

SDS-PAGE analysis of the purified antigen revealed that SmGST has molecular weight around 28 KD was used as a vaccine in a dose of 25 and 35 jig.

Eleven groups of BALB/c mice of 10 mice each were vaccinated by GST.

Complete Freund's adjuvant (CFA) and BCG vaccine were used as adjuvant.

Booster doses were given after 2 & 4 weeks.

Two weeks af¬ter the last dose of vaccine, mice were challenged by S.

mansion cercariae.

Blood samples were taken 7 weeks post infection for detection of IgGl, IgE and circulating antigens.

Then mice were sacrificed for histopathological study Of the liver.

Highly significant (p>0.001) increase in the mean optical density of IgGl& IgE in groups vaccinated by 35jig GST and CFA was demonstrated.

On the other hand, highly significant (P<0.001) decrease in circulating antigen, grnu-loma number and size in the same group.