Crystal Structure of Deinococcus radiodurans RecQ Helicase Catalytic Core Domain: The Interdomain Flexibility
Joint Authors
Chen, Sheng-Chia
Huang, Chi-Hung
Yang, Chia Shin
Way, Tzong-Der
Chen, Yeh
Chang, Ming-Chung
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
RecQ DNA helicases are key enzymes in the maintenance of genome integrity, and they have functions in DNA replication, recombination, and repair.
In contrast to most RecQs, RecQ from Deinococcus radiodurans (DrRecQ) possesses an unusual domain architecture that is crucial for its remarkable ability to repair DNA.
Here, we determined the crystal structures of the DrRecQ helicase catalytic core and its ADP-bound form, revealing interdomain flexibility in its first RecA-like and winged-helix (WH) domains.
Additionally, the WH domain of DrRecQ is positioned in a different orientation from that of the E.
coli RecQ (EcRecQ).
These results suggest that the orientation of the protein during DNA-binding is significantly different when comparing DrRecQ and EcRecQ.
American Psychological Association (APA)
Chen, Sheng-Chia& Huang, Chi-Hung& Yang, Chia Shin& Way, Tzong-Der& Chang, Ming-Chung& Chen, Yeh. 2014. Crystal Structure of Deinococcus radiodurans RecQ Helicase Catalytic Core Domain: The Interdomain Flexibility. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034436
Modern Language Association (MLA)
Chen, Sheng-Chia…[et al.]. Crystal Structure of Deinococcus radiodurans RecQ Helicase Catalytic Core Domain: The Interdomain Flexibility. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1034436
American Medical Association (AMA)
Chen, Sheng-Chia& Huang, Chi-Hung& Yang, Chia Shin& Way, Tzong-Der& Chang, Ming-Chung& Chen, Yeh. Crystal Structure of Deinococcus radiodurans RecQ Helicase Catalytic Core Domain: The Interdomain Flexibility. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034436
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034436