Early Changes in Costameric and Mitochondrial Protein Expression with Unloading Are Muscle Specific
Joint Authors
Li, Ruowei
Valdivieso, Paola
Linnehan, Richard M.
Castells, Josiane
Tesch, Per
Gustafsson, Thomas
Flück, Martin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We hypothesised that load-sensitive expression of costameric proteins, which hold the sarcomere in place and position the mitochondria, contributes to the early adaptations of antigravity muscle to unloading and would depend on muscle fibre composition and chymotrypsin activity of the proteasome.
Biopsies were obtained from vastus lateralis (VL) and soleus (SOL) muscles of eight men before and after 3 days of unilateral lower limb suspension (ULLS) and subjected to fibre typing and measures for costameric (FAK and FRNK), mitochondrial (NDUFA9, SDHA, UQCRC1, UCP3, and ATP5A1), and MHCI protein and RNA content.
Mean cross-sectional area (MCSA) of types I and II muscle fibres in VL and type I fibres in SOL demonstrated a trend for a reduction after ULLS (0.05≤P<0.10).
FAK phosphorylation at tyrosine 397 showed a 20% reduction in VL muscle (P=0.029).
SOL muscle demonstrated a specific reduction in UCP3 content (-23%; P = 0.012).
Muscle-specific effects of ULLS were identified for linear relationships between measured proteins, chymotrypsin activity and fibre MCSA.
The molecular modifications in costamere turnover and energy homoeostasis identify that aspects of atrophy and fibre transformation are detectable at the protein level in weight-bearing muscles within 3 days of unloading.
American Psychological Association (APA)
Flück, Martin& Li, Ruowei& Valdivieso, Paola& Linnehan, Richard M.& Castells, Josiane& Tesch, Per…[et al.]. 2014. Early Changes in Costameric and Mitochondrial Protein Expression with Unloading Are Muscle Specific. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034488
Modern Language Association (MLA)
Flück, Martin…[et al.]. Early Changes in Costameric and Mitochondrial Protein Expression with Unloading Are Muscle Specific. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1034488
American Medical Association (AMA)
Flück, Martin& Li, Ruowei& Valdivieso, Paola& Linnehan, Richard M.& Castells, Josiane& Tesch, Per…[et al.]. Early Changes in Costameric and Mitochondrial Protein Expression with Unloading Are Muscle Specific. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034488