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Type 2C Phosphatase 1 of Artemisia annua L. Is a Negative Regulator of ABA Signaling
Joint Authors
Wang, Guo-Feng
Zhang, Fangyuan
Fu, Xueqing
Lv, Zongyou
Shen, Qian
Yan, Tingxian
Jiang, Weiming
Sun, Xiaofen
Tang, Ke-Xuan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response.
Additionally, ABA also regulates secondary metabolism such as artemisinin in the medicinal plant Artemisia annua L.
Although an earlier study showed that ABA receptor, AaPYL9, plays a positive role in ABA-induced artemisinin content improvement, many components in the ABA signaling pathway remain to be elucidated in Artemisia annua L.
To get insight of the function of AaPYL9, we isolated and characterized an AaPYL9-interacting partner, AaPP2C1.
The coding sequence of AaPP2C1 encodes a deduced protein of 464 amino acids, with all the features of plant type clade A PP2C.
Transcriptional analysis showed that the expression level of AaPP2C1 is increased after ABA, salt, and drought treatments.
Yeast two-hybrid and bimolecular fluorescence complementation assays (BiFC) showed that AaPYL9 interacted with AaPP2C1.
The P89S, H116A substitution in AaPYL9 as well as G199D substitution or deletion of the third phosphorylation site-like motif in AaPP2C1 abolished this interaction.
Furthermore, constitutive expression of AaPP2C1 conferred ABA insensitivity compared with the wild type.
In summary, our data reveals that AaPP2C1 is an AaPYL9-interacting partner and involved in the negative modulation of the ABA signaling pathway in A.
annua L.
American Psychological Association (APA)
Zhang, Fangyuan& Fu, Xueqing& Lv, Zongyou& Shen, Qian& Yan, Tingxian& Jiang, Weiming…[et al.]. 2014. Type 2C Phosphatase 1 of Artemisia annua L. Is a Negative Regulator of ABA Signaling. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034490
Modern Language Association (MLA)
Zhang, Fangyuan…[et al.]. Type 2C Phosphatase 1 of Artemisia annua L. Is a Negative Regulator of ABA Signaling. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1034490
American Medical Association (AMA)
Zhang, Fangyuan& Fu, Xueqing& Lv, Zongyou& Shen, Qian& Yan, Tingxian& Jiang, Weiming…[et al.]. Type 2C Phosphatase 1 of Artemisia annua L. Is a Negative Regulator of ABA Signaling. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034490