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Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34+ Cell Determination
Joint Authors
Vokurkova, Doris
Fatorova, I.
Rezacova, V.
Zak, P.
Lánská, Miriam
Bláha, Milan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Hematopoietic stem cells (HSCs), still represent a certain mystery in biology, have a unique property of dividing into equal cells and repopulating the hematopoietic tissue.
This potential enables their use in transplantation treatments.
The quality of the HSC grafts for transplantation is evaluated by flow cytometric determination of the CD34+ cells, which enables optimal timing of the first apheresis and the acquisition of maximal yield of the peripheral blood stem cells (PBSCs).
To identify a more efficient method for evaluating CD34+ cells, we compared the following alternative methods with the reference method: hematopoietic progenitor cells (HPC) enumeration (using the Sysmex XE-2100 analyser), detection of CD133+ cells, and quantification of aldehyde dehydrogenase activity in the PBSCs.
266 aphereses (84 patients) were evaluated.
In the preapheretic blood, the new methods produced data that were in agreement with the reference method.
The ROC curves have shown that for the first-day apheresis target, the optimal predictive cut-off value was 0.032 cells/mL for the HPC method (sensitivity 73.4%, specificity 69.3%).
HPC method exhibited a definite practical superiority as compared to other methods tested.
HPC enumeration could serve as a supplementary method for the optimal timing of the first apheresis; it is simple, rapid, and cheap.
American Psychological Association (APA)
Fatorova, I.& Bláha, Milan& Lánská, Miriam& Vokurkova, Doris& Rezacova, V.& Zak, P.. 2014. Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34+ Cell Determination. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1034502
Modern Language Association (MLA)
Fatorova, I.…[et al.]. Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34+ Cell Determination. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1034502
American Medical Association (AMA)
Fatorova, I.& Bláha, Milan& Lánská, Miriam& Vokurkova, Doris& Rezacova, V.& Zak, P.. Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34+ Cell Determination. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1034502
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034502