The Step Further to Understand the Role of Cytosolic Phospholipase A2 Alpha and Group X Secretory Phospholipase A2 in Allergic Inflammation: Pilot Study
Joint Authors
Sokolowska, Milena
Kupryś-Lipińska, Izabela
Przybek, Monika
Kuna, Piotr
Pawliczak, Rafal
Pniewska, Ewa
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Allergens, viral, and bacterial infections are responsible for asthma exacerbations that occur with progression of airway inflammation.
cPLA2α and sPLA2X are responsible for delivery of arachidonic acid for production of eicosanoids—one of the key mediators of airway inflammation.
However, cPLA2α and sPLA2X role in allergic inflammation has not been fully elucidated.
The aim of this study was to analyze the influence of rDer p1 and rFel d1 and lipopolysaccharide (LPS) on cPLA2α expression and sPLA2X secretion in PBMC of asthmatics and in A549 cell line.
PBMC isolated from 14 subjects, as well as A549 cells, were stimulated with rDer p1, rFel d1, and LPS.
Immunoblotting technique was used to study the changes in cPLA2α protein expression and ELISA was used to analyze the release of sPLA2X.
PBMC of asthmatics released more sPLA2X than those from healthy controls in the steady state.
rDer p1 induced more sPLA2X secretion than cPLA2α protein expression.
rFel d1 caused decrease in cPLA2α relative expression in PBMC of asthmatics and in A549 cells.
Summarizing, Der p1 and Fel d1 involve phospholipase A2 enzymes in their action.
sPLA2X seems to be one of important PLA2 isoform in allergic inflammation, especially caused by house dust mite allergens.
American Psychological Association (APA)
Pniewska, Ewa& Sokolowska, Milena& Kupryś-Lipińska, Izabela& Przybek, Monika& Kuna, Piotr& Pawliczak, Rafal. 2014. The Step Further to Understand the Role of Cytosolic Phospholipase A2 Alpha and Group X Secretory Phospholipase A2 in Allergic Inflammation: Pilot Study. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034527
Modern Language Association (MLA)
Pniewska, Ewa…[et al.]. The Step Further to Understand the Role of Cytosolic Phospholipase A2 Alpha and Group X Secretory Phospholipase A2 in Allergic Inflammation: Pilot Study. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1034527
American Medical Association (AMA)
Pniewska, Ewa& Sokolowska, Milena& Kupryś-Lipińska, Izabela& Przybek, Monika& Kuna, Piotr& Pawliczak, Rafal. The Step Further to Understand the Role of Cytosolic Phospholipase A2 Alpha and Group X Secretory Phospholipase A2 in Allergic Inflammation: Pilot Study. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034527
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034527