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Silencing of Plasma Membrane Ca2+-ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells
Joint Authors
Boczek, Tomasz
Lisek, Malwina
Ferenc, Bozena
Kowalski, Antoni
Zylinska, Ludmila
Wiktorska, Magdalena
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-07
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
A close link between Ca2+, ATP level, and neurogenesis is apparent; however, the molecular mechanisms of this relationship have not been completely elucidated.
Transient elevations of cytosolic Ca2+ may boost ATP synthesis, but ATP is also consumed by ion pumps to maintain a low Ca2+ in cytosol.
In differentiation process plasma membrane Ca2+ ATPase (PMCA) is considered as one of the major players for Ca2+ homeostasis.
From four PMCA isoforms, the fastest PMCA2 and PMCA3 are expressed predominantly in excitable cells.
In the present study we assessed whether PMCA isoform composition may affect energy balance in differentiating PC12 cells.
We found that PMCA2-downregulated cells showed higher basal O2 consumption, lower NAD(P)H level, and increased activity of ETC.
These changes associated with higher [Ca2+]c resulted in elevated ATP level.
Since PMCA2-reduced cells demonstrated greatest sensitivity to ETC inhibition, we suppose that the main source of energy for PMCA isoforms 1, 3, and 4 was oxidative phosphorylation.
Contrary, cells with unchanged PMCA2 expression exhibited prevalence of glycolysis in ATP generation.
Our results with PMCA2- or PMCA3-downregulated lines provide an evidence of a novel role of PMCA isoforms in regulation of bioenergetic pathways, and mitochondrial activity and maintenance of ATP level during PC12 cells differentiation.
American Psychological Association (APA)
Boczek, Tomasz& Lisek, Malwina& Ferenc, Bozena& Kowalski, Antoni& Wiktorska, Magdalena& Zylinska, Ludmila. 2014. Silencing of Plasma Membrane Ca2+-ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1034551
Modern Language Association (MLA)
Boczek, Tomasz…[et al.]. Silencing of Plasma Membrane Ca2+-ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1034551
American Medical Association (AMA)
Boczek, Tomasz& Lisek, Malwina& Ferenc, Bozena& Kowalski, Antoni& Wiktorska, Magdalena& Zylinska, Ludmila. Silencing of Plasma Membrane Ca2+-ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1034551
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034551