Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Joint Authors
Wang, Wen-Ling
Sheu, Shi-Yuan
Fu, Yuan-Tsung
Kuo, Tzong-Fu
Kao, Shung-Te
Chen, Yueh-Sheng
Yao, Chun-Hsu
Chen, Kuo-Yu
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Large bone defects are a considerable challenge to reconstructive surgeons.
Numerous traditional Chinese herbal medicines have been used to repair and regenerate bone tissue.
This study investigated the bone regeneration potential of Danggui Buxue Tang (DBT), a Chinese herbal decoction prepared from Radix Astragali (RA) and Radix Angelicae Sinensis (RAS), from a molecular biology perspective.
The optimal ratio of RA and RAS used in DBT for osteoblast culture was obtained by colorimetric and alkaline phosphatase (ALP) activity assays.
Moreover, the optimal concentration of DBT for bone cell culture was also determined by colorimetric, ALP activity, nodule formation, Western blotting, wound-healing, and tartrate-resistant acid phosphatase activity assays.
Consequently, the most appropriate weight ratio of RA to RAS for the proliferation and differentiation of osteoblasts was 5 : 1.
Moreover, the most effective concentration of DBT was 1,000 μg/mL, which significantly increased the number of osteoblasts, intracellular ALP levels, and nodule numbers, while inhibiting osteoclast activity.
Additionally, 1,000 μg/mL of DBT was able to stimulate p-ERK and p-JNK signal pathway.
Therefore, DBT is highly promising for use in accelerating fracture healing in the middle or late healing periods.
American Psychological Association (APA)
Wang, Wen-Ling& Sheu, Shi-Yuan& Chen, Yueh-Sheng& Kao, Shung-Te& Fu, Yuan-Tsung& Kuo, Tzong-Fu…[et al.]. 2014. Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034584
Modern Language Association (MLA)
Wang, Wen-Ling…[et al.]. Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1034584
American Medical Association (AMA)
Wang, Wen-Ling& Sheu, Shi-Yuan& Chen, Yueh-Sheng& Kao, Shung-Te& Fu, Yuan-Tsung& Kuo, Tzong-Fu…[et al.]. Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034584
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034584