Synthesis and Preclinical Characterization of [18F]FPBZA: A Novel PET Probe for Melanoma
Joint Authors
Lin, Wuu-Jyh
Wu, Shih-Yen
Huang, Shih-Pin
Lo, Yen-Chen
Wang, Shyh-Jen
Shen, Chih-Chieh
Liu, Ren-Shyan
Wang, Hsin-Ell
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Introduction.
Benzamide can specifically bind to melanoma cells.
A 18F-labeled benzamide derivative, [18F]N-(2-diethylaminoethyl)-4-[2-(2-(2-fluoroethoxy) ethoxy)ethoxy]benzamide ([18F]FPBZA), was developed as a promising PET probe for primary and metastatic melanoma.
Methods.
[18F]FPBZA was synthesized via a one-step radiofluorination in this study.
The specific uptake of [18F]FPBZA was studied in B16F0 melanoma cells, A375 amelanotic melanoma cells, and NB-DNJ-pretreated B16F0 melanoma cells.
The biological characterization of [18F]FPBZA was performed on mice bearing B16F0 melanoma, A375 amelanotic melanoma, or inflammation lesion.
Results.
[18F]FPBZA can be prepared efficiently with a yield of 40–50%.
The uptake of [18F]FPBZA by B16F0 melanoma cells was significantly higher than those by A375 tumor cells and NB-DNJ-pretreated B16F0 melanoma cells.
B16F0 melanoma displayed prominent uptake of [18F]FPBZA at 2 h (7.81±0.82 %ID/g), compared with A375 tumor and inflammation lesion (3.00±0.71 and 1.67±0.56 %ID/g, resp.).
[18F]FPBZA microPET scan clearly delineated B16F0 melanoma but not A375 tumor and inflammation lesion.
In mice bearing pulmonary metastases, the lung radioactivity reached 4.77±0.36 %ID/g at 2 h (versus 1.16±0.23 %ID/g in normal mice).
Conclusions.
Our results suggested that [18F]FPBZA PET would provide a promising and specific approach for the detection of primary and metastatic melanoma lesions.
American Psychological Association (APA)
Wu, Shih-Yen& Huang, Shih-Pin& Lo, Yen-Chen& Liu, Ren-Shyan& Wang, Shyh-Jen& Lin, Wuu-Jyh…[et al.]. 2014. Synthesis and Preclinical Characterization of [18F]FPBZA: A Novel PET Probe for Melanoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034603
Modern Language Association (MLA)
Wu, Shih-Yen…[et al.]. Synthesis and Preclinical Characterization of [18F]FPBZA: A Novel PET Probe for Melanoma. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1034603
American Medical Association (AMA)
Wu, Shih-Yen& Huang, Shih-Pin& Lo, Yen-Chen& Liu, Ren-Shyan& Wang, Shyh-Jen& Lin, Wuu-Jyh…[et al.]. Synthesis and Preclinical Characterization of [18F]FPBZA: A Novel PET Probe for Melanoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034603
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034603