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Female Aging Alters Expression of Human Cumulus Cells Genes that Are Essential for Oocyte Quality
Joint Authors
Hamamah, Samir
Lecellier, Charles-Henri
Al-Edani, Tamadir
Ferrières, Alice
Bringer Deutsch, Sophie
Aït-Ahmed, Ounissa
Assou, Said
Gala, Anna
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Impact of female aging is an important issue in human reproduction.
There was a need for an extensive analysis of age impact on transcriptome profile of cumulus cells (CCs) to link oocyte quality and developmental potential with patient’s age.
CCs from patients of three age groups were analyzed individually using microarrays.
RT-qPCR validation was performed on independent CC cohorts.
We focused here on pathways affected by aging in CCs that may explain the decline of oocyte quality with age.
In CCs collected from patients >37 years, angiogenic genes including ANGPTL4, LEPR, TGFBR3, and FGF2 were significantly overexpressed compared to patients of the two younger groups.
In contrast genes implicated in TGF-β signaling pathway such as AMH, TGFB1, inhibin, and activin receptor were underexpressed.
CCs from patients whose ages are between 31 and 36 years showed an overexpression of genes related to insulin signaling pathway such as IGFBP3, PIK3R1, and IGFBP5.
A bioinformatic analysis was performed to identify the microRNAs that are potential regulators of the differentially expressed genes of the study.
It revealed that the pathways impacted by age were potential targets of specific miRNAs previously identified in our CCs small RNAs sequencing.
American Psychological Association (APA)
Al-Edani, Tamadir& Assou, Said& Ferrières, Alice& Bringer Deutsch, Sophie& Gala, Anna& Lecellier, Charles-Henri…[et al.]. 2014. Female Aging Alters Expression of Human Cumulus Cells Genes that Are Essential for Oocyte Quality. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034615
Modern Language Association (MLA)
Al-Edani, Tamadir…[et al.]. Female Aging Alters Expression of Human Cumulus Cells Genes that Are Essential for Oocyte Quality. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1034615
American Medical Association (AMA)
Al-Edani, Tamadir& Assou, Said& Ferrières, Alice& Bringer Deutsch, Sophie& Gala, Anna& Lecellier, Charles-Henri…[et al.]. Female Aging Alters Expression of Human Cumulus Cells Genes that Are Essential for Oocyte Quality. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034615
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034615