Computational Biophysical, Biochemical, and Evolutionary Signature of Human R-Spondin Family Proteins, the Member of Canonical Wntβ-Catenin Signaling Pathway
Joint Authors
Sharma, Ashish Ranjan
Yoon, Jeong Kyo
Song, Dong-Keun
Priya Doss, C. George
Chakraborty, Chiranjib
Nam, Ju-Suk
Lee, Sang-Soo
Sharma, Garima
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-08
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
In human, Wnt/β-catenin signaling pathway plays a significant role in cell growth, cell development, and disease pathogenesis.
Four human (Rspo)s are known to activate canonical Wnt/β-catenin signaling pathway.
Presently, (Rspo)s serve as therapeutic target for several human diseases.
Henceforth, basic understanding about the molecular properties of (Rspo)s is essential.
We approached this issue by interpreting the biochemical and biophysical properties along with molecular evolution of (Rspo)s thorough computational algorithm methods.
Our analysis shows that signal peptide length is roughly similar in (Rspo)s family along with similarity in aa distribution pattern.
In Rspo3, four N-glycosylation sites were noted.
All members are hydrophilic in nature and showed alike GRAVY values, approximately.
Conversely, Rspo3 contains the maximum positively charged residues while Rspo4 includes the lowest.
Four highly aligned blocks were recorded through Gblocks.
Phylogenetic analysis shows Rspo4 is being rooted with Rspo2 and similarly Rspo3 and Rspo1 have the common point of origin.
Through phylogenomics study, we developed a phylogenetic tree of sixty proteins (n=60) with the orthologs and paralogs seed sequences.
Protein-protein network was also illustrated.
Results demonstrated in our study may help the future researchers to unfold significant physiological and therapeutic properties of (Rspo)s in various disease models.
American Psychological Association (APA)
Sharma, Ashish Ranjan& Chakraborty, Chiranjib& Lee, Sang-Soo& Sharma, Garima& Yoon, Jeong Kyo& Priya Doss, C. George…[et al.]. 2014. Computational Biophysical, Biochemical, and Evolutionary Signature of Human R-Spondin Family Proteins, the Member of Canonical Wntβ-Catenin Signaling Pathway. BioMed Research International،Vol. 2014, no. 2014, pp.1-22.
https://search.emarefa.net/detail/BIM-1034617
Modern Language Association (MLA)
Sharma, Ashish Ranjan…[et al.]. Computational Biophysical, Biochemical, and Evolutionary Signature of Human R-Spondin Family Proteins, the Member of Canonical Wntβ-Catenin Signaling Pathway. BioMed Research International No. 2014 (2014), pp.1-22.
https://search.emarefa.net/detail/BIM-1034617
American Medical Association (AMA)
Sharma, Ashish Ranjan& Chakraborty, Chiranjib& Lee, Sang-Soo& Sharma, Garima& Yoon, Jeong Kyo& Priya Doss, C. George…[et al.]. Computational Biophysical, Biochemical, and Evolutionary Signature of Human R-Spondin Family Proteins, the Member of Canonical Wntβ-Catenin Signaling Pathway. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-22.
https://search.emarefa.net/detail/BIM-1034617
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034617