Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization
Joint Authors
Neelagund, S. E.
Iiger, Sudhir R.
Godbole, Ajeet M.
Kulkarni, Venkatrao
Vastrad, B. M.
Source
Biochemistry Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Optimization of various production parameters using response surface methodology (RSM) was performed to assess maximum yield of rifamycin B from Nocardia mediterranei MTCC 14.
Plackett-Burman design test was applied to determine the significant effects of various production parameters such as glucose, maltose, ribose, galactose, beef extract, peanut meal, ammonium chloride, ammonium sulphate, barbital, pH, and moisture content on production of rifamycin B.
Among the eleven variables tested, galactose, ribose, glucose, and pH were found to have significant effect on rifamycin B production.
Optimum levels of the significant variables were decided by using a central composite design.
The most appropriate condition for production of rifamycin B was found to be a single step production at galactose (8% w/w), ribose (3% w/w), glucose (9% w/w), and pH (7.0).
At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake.
The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.
American Psychological Association (APA)
Vastrad, B. M.& Neelagund, S. E.& Iiger, Sudhir R.& Godbole, Ajeet M.& Kulkarni, Venkatrao. 2014. Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization. Biochemistry Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1034629
Modern Language Association (MLA)
Vastrad, B. M.…[et al.]. Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization. Biochemistry Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1034629
American Medical Association (AMA)
Vastrad, B. M.& Neelagund, S. E.& Iiger, Sudhir R.& Godbole, Ajeet M.& Kulkarni, Venkatrao. Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization. Biochemistry Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1034629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034629