Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERKp38 MAPK Pathways
Joint Authors
Ouyang, Qiufang
Han, Ying
Lin, Zhi-Hong
Xie, Hong
Xu, Chang-Sheng
Xie, Liang-Di
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Abnormal phenotypic switch of vascular smooth muscle cell (VSMC) is a hallmark of vascular disorders such as atherosclerosis and restenosis.
And this process has been related to remodeling of L-type calcium channel (LTCC).
We attempted to investigate whether fluvastatin has any effect on VSMC proliferation and LTCC α 1 C subunit ( L T C C α 1 C ) expression as well as the potential mechanisms involved.
The VSMCs proliferation was assayed by osteopontin immunofluorescent staining and [3H]-thymidine incorporation.
The cell cycle was detected by flow cytometric analysis.
The activity of RhoA was determined with pull-down assay.
MAPK activity and L T C C α 1 C expression were assessed by western blotting.
We demonstrated fluvastatin prevented the VSMCs dedifferentiating into a proliferative phenotype and induced cell cycle arrest in the G0/G1 phase in response to PDGF-BB stimulation.
Fluvastatin dose-dependently reversed the downregulation of L T C C α 1 C expression induced by PDGF-BB.
Inhibition of ROCK, ERK, or p38 MAPK activation largely enhanced the upregulation effect of fluvastatin ( P < 0.01 ) .
However, blockade of JNK pathway had no effect on L T C C α 1 C expression.
We concluded L T C C α 1 C was a VSMC contractile phenotype marker gene.
Fluvastatin upregulated L T C C α 1 C expression, at least in part, by inhibiting ROCK, ERK1/2, and p38 MAPK activation.
Fluvastatin may be a potential candidate for preventing or treating vascular diseases.
American Psychological Association (APA)
Ouyang, Qiufang& Han, Ying& Lin, Zhi-Hong& Xie, Hong& Xu, Chang-Sheng& Xie, Liang-Di. 2014. Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERKp38 MAPK Pathways. Disease Markers،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1035051
Modern Language Association (MLA)
Ouyang, Qiufang…[et al.]. Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERKp38 MAPK Pathways. Disease Markers No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1035051
American Medical Association (AMA)
Ouyang, Qiufang& Han, Ying& Lin, Zhi-Hong& Xie, Hong& Xu, Chang-Sheng& Xie, Liang-Di. Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERKp38 MAPK Pathways. Disease Markers. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1035051
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035051