Mulberry Extracts Alleviate Aβ 25–35-Induced Injury and Change the Gene Expression Profile in PC12 Cells
Joint Authors
Song, Nan
Yang, Hongpeng
Pang, Wei
Qie, Zhiwei
Lu, Hao
Tan, Long
Li, Haiqiang
Sun, Shoudan
Lian, Fuzhi
Jiang, Yugang
Qin, Chuan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Mulberry, which contained high amounts of anthocyanins, has been used in traditional Chinese medicine.
Mulberry fruit extracts (ME) have demonstrated the antioxidant activity and neuroprotection.
The study was to investigate the neuroprotective efficacy of ME against β-amyloid 25–35- (Aβ 25–35-) induced PC12 cells injury.
Cells preincubated with or without ME (200 μg/mL) for 24 h were treated with Aβ 25–35 (20 μmol/L) for another 24 h.
Cell viability was assessed by MTT, gene expression profiles were examined by cDNA microarrays, and RT-PCR were used to confirm the results of microarray assays.
ME pretreatment was found to neutralize the cytotoxicity and prevent Aβ 25–35-induced cells injury.
Analyses of gene expression profile revealed that genes involving cell adhesion, peptidase activity, cytokine activity, ion binding activity, and angiogenesis regulation were significantly modulated by ME pretreatment.
Among those genes, Apaf1, Bace2, and Plcb4 were enriched in the “Alzheimer’s disease-reference pathway” and downregulated after ME intervention.
RT-PCR results showed that ME preincubation could significantly inhibit Aβ 25–35 increased mRNA levels of these three genes.
Overall, ME pretreatment could substantially alleviate PC12 cells injury and downregulate expression of AD-related genes, such as Apaf1, Bace2, and Plcb4.
This study has a great nutrigenomics interest and brings new and important light in the field of AD intervention.
American Psychological Association (APA)
Song, Nan& Yang, Hongpeng& Pang, Wei& Qie, Zhiwei& Lu, Hao& Tan, Long…[et al.]. 2014. Mulberry Extracts Alleviate Aβ 25–35-Induced Injury and Change the Gene Expression Profile in PC12 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1035106
Modern Language Association (MLA)
Song, Nan…[et al.]. Mulberry Extracts Alleviate Aβ 25–35-Induced Injury and Change the Gene Expression Profile in PC12 Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1035106
American Medical Association (AMA)
Song, Nan& Yang, Hongpeng& Pang, Wei& Qie, Zhiwei& Lu, Hao& Tan, Long…[et al.]. Mulberry Extracts Alleviate Aβ 25–35-Induced Injury and Change the Gene Expression Profile in PC12 Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1035106
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035106