Antitumor Activity of Chinese Propolis in Human Breast Cancer MCF-7 and MDA-MB-231 Cells
Joint Authors
Wang, Kai
Xuan, Hongzhuan
Li, Zhen
Yan, Haiyue
Sang, Qing
He, Qingtao
Wang, Yuanjun
Hu, Fuliang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Chinese propolis has been reported to possess various biological activities such as antitumor.
In present study, anticancer activity of ethanol extract of Chinese propolis (EECP) at 25, 50, 100, and 200 μg/mL was explored by testing the cytotoxicity in MCF-7 (human breast cancer ER(+)) and MDA-MB-231 (human breast cancer ER(−)) cells.
EECP revealed a dose- and time-dependent cytotoxic effect.
Furthermore, annexin A7 (ANXA7), p53, nuclear factor-κB p65 (NF-κB p65), reactive oxygen species (ROS) levels, and mitochondrial membrane potential were investigated.
Our data indicated that treatment of EECP for 24 and 48 h induced both cells apoptosis obviously.
Exposure to EECP significantly increased ANXA7 expression and ROS level, and NF-κB p65 level and mitochondrial membrane potential were depressed by EECP dramatically.
The effects of EECP on p53 level were different in MCF-7 and MDA-MB-231 cells, which indicated that EECP exerted its antitumor effects in MCF-7 and MDA-MB-231 cells by inducing apoptosis, regulating the levels of ANXA7, p53, and NF-κB p65, upregulating intracellular ROS, and decreasing mitochondrial membrane potential.
Interestingly, EECP had little or small cytotoxicity on normal human umbilical vein endothelial cells (HUVECs).
These results suggest that EECP is a potential alternative agent on breast cancer treatment.
American Psychological Association (APA)
Xuan, Hongzhuan& Li, Zhen& Yan, Haiyue& Sang, Qing& Wang, Kai& He, Qingtao…[et al.]. 2014. Antitumor Activity of Chinese Propolis in Human Breast Cancer MCF-7 and MDA-MB-231 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035138
Modern Language Association (MLA)
Xuan, Hongzhuan…[et al.]. Antitumor Activity of Chinese Propolis in Human Breast Cancer MCF-7 and MDA-MB-231 Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1035138
American Medical Association (AMA)
Xuan, Hongzhuan& Li, Zhen& Yan, Haiyue& Sang, Qing& Wang, Kai& He, Qingtao…[et al.]. Antitumor Activity of Chinese Propolis in Human Breast Cancer MCF-7 and MDA-MB-231 Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035138
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035138