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Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1 β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice
Joint Authors
Liang, Chan-Jung
Lee, Chiang-Wen
Sung, Hsin-Ching
Li, Chi-Yuan
Chiang, Yao-Chang
Hsu, Hsien-Yeh
Tseng, Ying-Chin
Wang, Shu-Huei
Chen, Yung-Hsiang
Chen, Yuh-Lien
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-04
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The expression of inflammatory cytokines on vascular walls is a critical event in vascular diseases and inflammation.
The aim of the present study was to examine the effects of an extract of Ganoderma lucidum (Reishi) polysaccharides (EORPs), which is effective against immunological disorders, on interleukin- (IL-) 1β expression by human aortic smooth muscle cells (HASMCs) and the underlying mechanism.
The lipopolysaccharide- (LPS-) induced IL-1β expression was significantly reduced when HASMCs were pretreated with EORP by Western blot and immunofluorescent staining.
Pretreatment with 10 μg/mL EORP decreased LPS-induced ERK, p38, JNK, and Akt phosphorylation.
But the increase in IL-1β expression with LPS treatment was only inhibited by pretreatment with the ERK1/2 inhibitor, while the JNK and p38 inhibitors had no effect.
In addition, EORP reduced the phosphorylation and nuclear translocation of nuclear factor- (NF-) κB p65 in LPS-treated HASMCs.
Furthermore, in vivo, IL-1β expression was strongly expressed in thoracic aortas in LPS-treated mice.
Oral administration of EORP decreased IL-1β expression.
The level of IL-1β expression in LPS-treated or in LPS/EORP-treated group was very low and was similar to that of the saline-treated group in toll-like receptor 4-deficient (TLR4−/−) mice.
These findings suggest that EORP has the anti-inflammatory property and could prove useful in the prevention of vascular diseases and inflammatory responses.
American Psychological Association (APA)
Liang, Chan-Jung& Lee, Chiang-Wen& Sung, Hsin-Ching& Chen, Yung-Hsiang& Chiang, Yao-Chang& Hsu, Hsien-Yeh…[et al.]. 2014. Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1 β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1035149
Modern Language Association (MLA)
Liang, Chan-Jung…[et al.]. Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1 β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1035149
American Medical Association (AMA)
Liang, Chan-Jung& Lee, Chiang-Wen& Sung, Hsin-Ching& Chen, Yung-Hsiang& Chiang, Yao-Chang& Hsu, Hsien-Yeh…[et al.]. Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1 β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1035149
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035149