Rhein Induces a Necrosis-Apoptosis Switch in Pancreatic Acinar Cells
Joint Authors
Zhu, Shi-Feng
Zhao, Xian-Lin
Wan, Mei-Hua
Tang, Wen-Fu
Li, Juan
Liu, Yiling
Zhao, Jianlei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
The Chinese herbal medicine Da-Cheng-Qi decoction can regulate a necrosis-apoptosis switch in injured pancreatic acinar cells.
This study investigated the effects of rhein, a component of this medicine, on a necrosis-apoptosis switch in pancreatic rat AR42J cells.
Methods.
Cerulein-treated AR42J cells were used.
After pretreatment with 479, 119.8, or 29.9 μg/L rhein, cells were cocultured with rhein and cerulein (10−8 M) for 4, 8, or 16 h.
Apoptosis and necrosis were examined using annexin V and propidium iodide costaining.
Mitochondria-dependent apoptosis-associated proteins were examined using enzyme-linked immunosorbent assays and western blotting.
Results.
Few cells died in untreated samples.
The number was significantly higher in 16-h-cerulein-treated samples and treatment with 479 μg/L rhein most effectively increased the apoptotic-to-necrotic cell ratio ( P < 0.05 ).
In cerulein-treated cells, rhein increased the concentrations of p53, cytochrome C, and caspase-3, and increased the Bax/Bcl-2 ratio in a time- and dose-dependent manner, with the maximum effect in cells treated with 479 μg/L rhein for 16 h ( P < 0.05 ).
Conclusions.
Rhein induces the necrosis-apoptosis switch in injured pancreatic acinar cells in a time- and dose-dependent manner.
Mitochondria-dependent apoptosis signaling pathways might play an important role in this effect.
American Psychological Association (APA)
Zhao, Xian-Lin& Li, Juan& Zhu, Shi-Feng& Liu, Yiling& Zhao, Jianlei& Wan, Mei-Hua…[et al.]. 2014. Rhein Induces a Necrosis-Apoptosis Switch in Pancreatic Acinar Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1035186
Modern Language Association (MLA)
Zhao, Xian-Lin…[et al.]. Rhein Induces a Necrosis-Apoptosis Switch in Pancreatic Acinar Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1035186
American Medical Association (AMA)
Zhao, Xian-Lin& Li, Juan& Zhu, Shi-Feng& Liu, Yiling& Zhao, Jianlei& Wan, Mei-Hua…[et al.]. Rhein Induces a Necrosis-Apoptosis Switch in Pancreatic Acinar Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1035186
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035186