Indntification of Egyptian fasciola species by pcr and re-striction endonucleases digestion of the nuculear Samall subunit ribosomal rna gene

Abstract EN

Fascioliasis is one of the familial- zoometric health problems of worldwide dis¬tribution including Egypt.

In this study, a simple and rapid polymerase chain reaction/restriction fragment length polymorphisms (PCR/RFLPs) assay, using the common restriction end nucleases Aval, EcoRI, Easel, Saclf and Avail was applied to differentiate between both Fasciola gigantica and F.

hepatica.

The five restriction end nucleases were used to differentiate between the two spe¬cies of Fasciola based on -1950 bp long sequence of the 18S nuclear small subunit ribosomal RNA gene.

Aval and EcpRI restriction end nucleases failed to differentiate between the two Fasciola species when each restriction enzyme gave the same restriction patterns in both of them.

However, F.

gigantica and F.

hepatica were well-differentiated when their small subunit ribosomal DNA were digested with Eael and Sacl 1 restriction end nucleases.