Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular-Signal-Regulated Kinase and Akt
Joint Authors
Yang, Won-Kyung
Kim, Seung-Hyung
Rhee, Man Hee
Kim, Dong-Seon
Kim, Ho Kyoung
Sung, Yoon-Young
Ji, Hyun Dong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Ethnopharmacological Relevance.
Morus alba L.
leaves (MAE) have been used in fork medicine for the treatment of beriberi, edema, diabetes, hypertension, and atherosclerosis.
However, underlying mechanism of MAE on cardiovascular protection remains to be elucidated.
Therefore, we investigated whether MAE affect platelet aggregation and thrombosis.
Materials and Methods.
The anti-platelet activity of MAE was studied using rat platelets.
The extent of anti-platelet activity of MAE was assayed in collagen-induced platelet aggregation.
ATP and serotonin release was carried out.
The activation of integrin α IIb β 3 and phosphorylation of signaling molecules, including MAPK and Akt, were investigated with cytofluorometer and immunoblotting, respectively.
The thrombus formation in vivo was also evaluated in arteriovenous shunt model of rats.
Results.
HPLC chromatographic analysis revealed that MAE contained rutin and isoquercetin.
MAE dose-dependently inhibited collagen-induced platelet aggregation.
MAE also attenuated serotonin secretion and thromboxane A2 formation.
In addition, the extract in vivo activity showed that MAE at 100, 200, and 400 mg/kg significantly and dose-dependently attenuated thrombus formation in rat arterio-venous shunt model by 52.3% ( P < 0 .001 ), 28.3% ( P < 0.01 ), and 19.1% ( P < 0.05 ), respectively.
Conclusions.
MAE inhibit platelet activation, TXB2 formation, serotonin secretion, aggregation, and thrombus formation.
The plant extract could be considered as a candidate to anti-platelet and antithrombotic agent.
American Psychological Association (APA)
Kim, Dong-Seon& Ji, Hyun Dong& Rhee, Man Hee& Sung, Yoon-Young& Yang, Won-Kyung& Kim, Seung-Hyung…[et al.]. 2014. Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular-Signal-Regulated Kinase and Akt. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035315
Modern Language Association (MLA)
Kim, Dong-Seon…[et al.]. Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular-Signal-Regulated Kinase and Akt. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1035315
American Medical Association (AMA)
Kim, Dong-Seon& Ji, Hyun Dong& Rhee, Man Hee& Sung, Yoon-Young& Yang, Won-Kyung& Kim, Seung-Hyung…[et al.]. Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular-Signal-Regulated Kinase and Akt. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035315
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035315