Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells
Joint Authors
Pêgas Henriques, João Antonio
Begnini, Karine Rech
Moura de Leon, Priscila Marques
Thurow, Helena
Schultze, Eduarda
Campos, Vinicius Farias
Martins Rodrigues, Fernanda
Borsuk, Sibele
Savegnago, Lucielli
Roesch-Ely, Mariana
Moura, Sidnei
Padilha, Francine F.
Seixas, Fabiana K.
Collares, Tiago
Dellagostin, Odir A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-03
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Natural products continue to be an invaluable resource of anticancer drug discovery in recent years.
Propolis is known for its biological activities such as antimicrobial and antitumor effects.
This study assessed the effects of Brazilian red propolis (BRP) on apoptosis and migration potential in human bladder cancer cells.
The effect of BRP ethanolic extract (25, 50, and 100 μg/mL) on 5637 cells was determined by MTT, LIVE/DEAD, and migration (scratch assay) assays.
Apoptosis induction was investigated through flow cytometry and gene expression profile was investigated by qRT-PCR.
Results showed cytotoxicity on MTT and LIVE/DEAD assays, with IC50 values of 95 μg/mL in 24 h of treatment.
Cellular migration of 5637 cells was significantly inhibited through lower doses of BRP ethanolic extract (25 and 50 μg/mL).
Flow cytometry analyses showed that BRP induced cytotoxicity through apoptosis-like mechanisms in 5637 cells and qRT-PCR revealed increased levels of Bax/Bcl-2 ratio, p53, AIF, and antioxidant enzymes genes.
Data suggest that BRP may be a potential source of drugs to bladder cancer treatment.
American Psychological Association (APA)
Begnini, Karine Rech& Moura de Leon, Priscila Marques& Thurow, Helena& Schultze, Eduarda& Campos, Vinicius Farias& Martins Rodrigues, Fernanda…[et al.]. 2014. Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1035316
Modern Language Association (MLA)
Begnini, Karine Rech…[et al.]. Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1035316
American Medical Association (AMA)
Begnini, Karine Rech& Moura de Leon, Priscila Marques& Thurow, Helena& Schultze, Eduarda& Campos, Vinicius Farias& Martins Rodrigues, Fernanda…[et al.]. Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1035316
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035316