Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding
Joint Authors
Huang, Lin-Fang
Chen, Shi-Lin
Zheng, Sihao
Liu, Dewang
Ren, Weiguang
Fu, Juan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Radix Astragali is a popular herb used in traditional Chinese medicine for its proimmune and antidiabetic properties.
However, methods are needed to help distinguish Radix Astragali from its varied adulterants.
DNA barcoding is a widely applicable molecular method used to identify medicinal plants.
Yet, its use has been hampered by genetic distance, base variation, and limitations of the bio-NJ tree.
Herein, we report the validation of an integrated analysis method for plant species identification using DNA barcoding that focuses on genetic distance, identification efficiency, inter- and intraspecific variation, and barcoding gap.
We collected 478 sequences from six candidate DNA barcodes (ITS2, ITS, psbA-trnH, rbcL, matK, and COI) from 29 species of Radix Astragali and adulterants.
The internal transcribed spacer (ITS) sequence was demonstrated as the optimal barcode for identifying Radix Astragali and its adulterants.
This new analysis method is helpful in identifying Radix Astragali and expedites the utilization and data mining of DNA barcoding.
American Psychological Association (APA)
Zheng, Sihao& Liu, Dewang& Ren, Weiguang& Fu, Juan& Huang, Lin-Fang& Chen, Shi-Lin. 2014. Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035412
Modern Language Association (MLA)
Zheng, Sihao…[et al.]. Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1035412
American Medical Association (AMA)
Zheng, Sihao& Liu, Dewang& Ren, Weiguang& Fu, Juan& Huang, Lin-Fang& Chen, Shi-Lin. Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035412
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035412