Proapoptotic and Antiproliferative Effects of Thymus caramanicus on Human Breast Cancer Cell Line (MCF-7) and Its Interaction with Anticancer Drug Vincristine
Joint Authors
Esmaeili-Mahani, Saeed
Falahi, Farzaneh
Yaghoobi, Mohammad Mehdi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Thymus caramanicus Jalas is one of the species of thymus that grows in the wild in different regions of Iran.
Traditionally, leaves of this plant are used in the treatment of diabetes, arthritis, and cancerous situation.
Therefore, the present study was designed to investigate the selective cytotoxic and antiproliferative properties of Thymus caramanicus extract (TCE).
MCF-7 human breast cancer cells were used in this study.
Cytotoxicity of the extract was determined using MTT and neutral red assays.
Biochemical markers of apoptosis (caspase 3, Bax, and Bcl-2) and cell proliferation (cyclin D1) were evaluated by immunoblotting.
Vincristine was used as anticancer control drug in extract combination therapy.
The data showed that incubation of cells with TCE (200 and 250 μg/mL) significantly increased cell damage, activated caspase 3 and Bax/Bcl2 ratio.
In addition, cyclin D1 was significantly decreased in TCE-treated cells.
Furthermore, concomitant treatment of cells with extract and anticancer drug produced a significant cytotoxic effect as compared to extract or drugs alone.
In conclusion, thymus extract has a potential proapoptotic/antiproliferative property against human breast cancer cells and its combination with chemotherapeutic agent vincristine may induce cell death effectively and be a potent modality to treat this type of cancer.
American Psychological Association (APA)
Esmaeili-Mahani, Saeed& Falahi, Farzaneh& Yaghoobi, Mohammad Mehdi. 2014. Proapoptotic and Antiproliferative Effects of Thymus caramanicus on Human Breast Cancer Cell Line (MCF-7) and Its Interaction with Anticancer Drug Vincristine. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1035439
Modern Language Association (MLA)
Esmaeili-Mahani, Saeed…[et al.]. Proapoptotic and Antiproliferative Effects of Thymus caramanicus on Human Breast Cancer Cell Line (MCF-7) and Its Interaction with Anticancer Drug Vincristine. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1035439
American Medical Association (AMA)
Esmaeili-Mahani, Saeed& Falahi, Farzaneh& Yaghoobi, Mohammad Mehdi. Proapoptotic and Antiproliferative Effects of Thymus caramanicus on Human Breast Cancer Cell Line (MCF-7) and Its Interaction with Anticancer Drug Vincristine. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1035439
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035439