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The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA
Joint Authors
Khoza, B. S.
Chimuka, L.
Mukwevho, E.
Steenkamp, P. A.
Madala, N. E.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Metabolite extraction methods have been shown to be a critical consideration for pharmacometabolomics studies and, as such, optimization and development of new extraction methods are crucial.
In the current study, an organic solvent-free method, namely, pressurised hot water extraction (PHWE), was used to extract pharmacologically important metabolites from dried Moringa oleifera leaves.
Here, the temperature of the extraction solvent (pure water) was altered while keeping other factors constant using a homemade PHWE system.
Samples extracted at different temperatures (50, 100, and 150°C) were assayed for antioxidant activities and the effect of the temperature on the extraction process was evaluated.
The samples were further analysed by mass spectrometry to elucidate their metabolite compositions.
Principal component analysis (PCA) evaluation of the UPLC-MS data showed distinctive differential metabolite patterns.
Here, temperature changes during PHWE were shown to affect the levels of metabolites with known pharmacological activities, such as chlorogenic acids and flavonoids.
Our overall findings suggest that, if not well optimised, the extraction temperature could compromise the “pharmacological potency” of the extracts.
The use of MS in combination with PCA was furthermore shown to be an excellent approach to evaluate the quality and content of pharmacologically important extracts.
American Psychological Association (APA)
Khoza, B. S.& Chimuka, L.& Mukwevho, E.& Steenkamp, P. A.& Madala, N. E.. 2014. The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1035451
Modern Language Association (MLA)
Khoza, B. S.…[et al.]. The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1035451
American Medical Association (AMA)
Khoza, B. S.& Chimuka, L.& Mukwevho, E.& Steenkamp, P. A.& Madala, N. E.. The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1035451
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035451