Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1
Joint Authors
Lu, Na-na
Liu, Qi
Gu, Li-gang
Ge, Shi-jie
Wu, Jun
Ze-ji, Qiu
Qiu, Ze-ji
Zhang, Hong-chun
Chao, En-xiang
Yu, Zhuo-nan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-08
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
A Chinese medicine granule, Shu-Feng-Xuan-Fei (SFXF), is critical for viral clearance in early phase of influenza virus infection.
In this study, 72 ICR mice were randomly divided into six groups: normal control group, virus control group, Oseltamivir group, low-dose SFXF, medium-dose SFXF, and high-dose SFXF.
Mice were anesthetized and inoculated with 4LD50 of influenza virus A (H1N1) except normal control group.
Oseltamivir group received 11.375 mg·kg−1·d−1 Oseltamivir Phosphate.
SFXF 3.76, 1.88 and 0.94 g·kg−1·d−1 were administrated to mice in all SFXF groups.
Each group was in equal dose of 0.2ml daily for 4 consecutive days.
Mice were sacrificed and then total RNA was extracted in lung tissue.
Some genes involved in T-cell-mediated immunity were selected by DNA microarray.
These candidate genes were verified by Real-Time PCR and western immunoblotting.
Compared with virus control group, in Toll-like receptor signaling pathway, 12 virus-altered genes were significantly reduced following medium-dose SFXF treatment.
Eighteen antigen processing presentation-associated genes were upregulated by medium-dose SFXF.
In the process of T cell receptor signaling pathway, 19 genes were downregulated by medium-dose SFXF treatment.
On exploration into effector T cells activation and cytokines, all of altered genes in virus control group were reversed by medium-dose SFXF.
Real-time PCR and western immunoblotting showed that the regulation of medium-dose SFXF in IL-4, IFN- γ , TNF- α , IL-1 β , TLR7, MyD88, p38, and JNK was superior to Oseltamivir and high-dose SFXF group.
Therefore, SFXF granules could reduce influenza infected cells and activation of T cells.
American Psychological Association (APA)
Lu, Na-na& Liu, Qi& Gu, Li-gang& Ge, Shi-jie& Wu, Jun& Ze-ji, Qiu…[et al.]. 2014. Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1035514
Modern Language Association (MLA)
Lu, Na-na…[et al.]. Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1035514
American Medical Association (AMA)
Lu, Na-na& Liu, Qi& Gu, Li-gang& Ge, Shi-jie& Wu, Jun& Ze-ji, Qiu…[et al.]. Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1035514
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035514