Pharmacokinetics and Tissue Distribution Study of Chlorogenic Acid from Lonicerae Japonicae Flos Following Oral Administrations in Rats
Joint Authors
Zhou, Yulu
Zhou, Ting
Pei, Qi
Liu, Shikun
Yuan, Hong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Chlorogenic acid (ChA) is proposed as the major bioactive compounds of Lonicerae Japonicae Flos (LJF).
Forty-two Wistar rats were randomly divided into seven groups to investigate the pharmacokinetics and tissue distribution of ChA, via oral administration of LJF extract, using ibuprofen as internal standard, employing a high performance liquid chromatography in conjunction with tandem mass spectrometry.
Analytes were extracted from plasma samples and tissue homogenate by liquid–liquid extraction with acetonitrile, separated on a C 18 column by linear gradient elution, and detected by electrospray ionization mass spectrometry in negative selected multiple reaction monitoring mode.
Our results successfully demonstrate that the method has satisfactory selectivity, linearity, extraction recovery, matrix effect, precision, accuracy, and stability.
Using noncompartment model to study pharmacokinetics, profile revealed that ChA was rapidly absorbed and eliminated.
Tissue study indicated that the highest level was observed in liver, followed by kidney, lung, heart, and spleen.
In conclusion, this method was suitable for the study on pharmacokinetics and tissue distribution of ChA after oral administration.
American Psychological Association (APA)
Zhou, Yulu& Zhou, Ting& Pei, Qi& Liu, Shikun& Yuan, Hong. 2014. Pharmacokinetics and Tissue Distribution Study of Chlorogenic Acid from Lonicerae Japonicae Flos Following Oral Administrations in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1035519
Modern Language Association (MLA)
Zhou, Yulu…[et al.]. Pharmacokinetics and Tissue Distribution Study of Chlorogenic Acid from Lonicerae Japonicae Flos Following Oral Administrations in Rats. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1035519
American Medical Association (AMA)
Zhou, Yulu& Zhou, Ting& Pei, Qi& Liu, Shikun& Yuan, Hong. Pharmacokinetics and Tissue Distribution Study of Chlorogenic Acid from Lonicerae Japonicae Flos Following Oral Administrations in Rats. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1035519
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035519