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Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through Gβγ-Mediated Pathway
Joint Authors
Jang, Hyeung-Jin
Shin, Min-Hee
Choi, Eun-Kyeong
Kim, Ki-Suk
Kim, Kang-Hoon
Chung, Won-Seok
Cha, Nam Hyun
Ahn, Kwang Seok
Jang, Young Pyo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Bupleurum falcatum L.
has been used traditionally as a medicinal herb in Korean medicine.
The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly.
We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF.
Through the microarray analysis, it was found that the ITPR2 gene that encodes the inositol 1,4,5-trisphosphate (IP3) receptor is up-regulated and the HFBF induces cell depolarization by inhibiting the voltage-gated channel expression in NCI-H716 cells.
In addition, we found that the intracellular calcium in NCI-H716 cells, with Gallein, U73122, and 2APB as inhibitors, was decreased.
These results suggest that the HFBF activates the GLP-1 secretion through the Gβγ pathways in the enteroendocrine L cells after treatment with the HFBF.
American Psychological Association (APA)
Shin, Min-Hee& Choi, Eun-Kyeong& Kim, Ki-Suk& Kim, Kang-Hoon& Jang, Young Pyo& Ahn, Kwang Seok…[et al.]. 2014. Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through Gβγ-Mediated Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1035523
Modern Language Association (MLA)
Shin, Min-Hee…[et al.]. Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through Gβγ-Mediated Pathway. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1035523
American Medical Association (AMA)
Shin, Min-Hee& Choi, Eun-Kyeong& Kim, Ki-Suk& Kim, Kang-Hoon& Jang, Young Pyo& Ahn, Kwang Seok…[et al.]. Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through Gβγ-Mediated Pathway. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1035523
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035523