![](/images/graphics-bg.png)
Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination
Joint Authors
Nafees, Muhammad
Asgher, Muhammad
Yaqoob, Mohammad
Nabi, Abdul
Siddiqi, Abdul Rauf
Murtaza, Ghulam
Source
International Journal of Analytical Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II) for the determination of vitamins A and C in pharmaceutical formulations.
The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB•−) which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2•−) and hydrogen peroxide (H2O2).
Highly reactive hydroxyl radicals (•OH) were produced via dismutation of H2O2 by catalyst (Cu2+).
The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence.
The limit of detection (3s of the blank, n=6) of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05 μg mL−1, respectively.
The sample throughput of 70 h−1 for vitamins A and C and 30 h−1 for RB was found.
Calibration curve was linear in the range of 0.05–15, 0.01–20, and 0.1–50 μg mL−1 for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; n=3) in the range 1.6–3.6%.
The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values.
American Psychological Association (APA)
Asgher, Muhammad& Yaqoob, Mohammad& Nabi, Abdul& Murtaza, Ghulam& Siddiqi, Abdul Rauf& Nafees, Muhammad. 2014. Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination. International Journal of Analytical Chemistry،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1035926
Modern Language Association (MLA)
Asgher, Muhammad…[et al.]. Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination. International Journal of Analytical Chemistry No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1035926
American Medical Association (AMA)
Asgher, Muhammad& Yaqoob, Mohammad& Nabi, Abdul& Murtaza, Ghulam& Siddiqi, Abdul Rauf& Nafees, Muhammad. Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination. International Journal of Analytical Chemistry. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1035926
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035926