Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides
Joint Authors
Gothwal, Ashish
Beniwal, Puneet
Dhull, Vikas
Hooda, Vikas
Source
International Journal of Analytical Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Polyvinyl chloride (PVC) can be used to develop reaction beaker which acts as electrochemical cell for the measurement of OP pesticides.
Being chemically inert, corrosion resistant, and easy in molding to various shapes and size, PVC can be used for the immobilization of enzyme.
Organophosphorus hydrolase was immobilized covalently onto the chemically activated inner surface of PVC beaker by using glutaraldehyde as a coupling agent.
The carbon nanotubes paste working electrode was constructed for amperometric measurement at a potential of +0.8 V.
The biosensor showed optimum response at pH 8.0 with incubation temperature of 40°C.
K m and I m a x for substrate (methyl parathion) were 322.58 µM and 1.1 µA, respectively.
Evaluation study showed a correlation of 0.985, which was in agreement with the standard method.
The OPH biosensor lost 50% of its initial activity after its regular use for 25 times over a period of 50 days when stored in 0.1 M sodium phosphate buffer, pH 8.0 at 4°C.
No interference was observed by interfering species.
American Psychological Association (APA)
Gothwal, Ashish& Beniwal, Puneet& Dhull, Vikas& Hooda, Vikas. 2014. Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides. International Journal of Analytical Chemistry،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1035931
Modern Language Association (MLA)
Gothwal, Ashish…[et al.]. Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides. International Journal of Analytical Chemistry No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1035931
American Medical Association (AMA)
Gothwal, Ashish& Beniwal, Puneet& Dhull, Vikas& Hooda, Vikas. Preparation of Electrochemical Biosensor for Detection of Organophosphorus Pesticides. International Journal of Analytical Chemistry. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1035931
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035931