High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function
Joint Authors
Zha, Min
Xu, Wei
Zhai, Qing
Li, Fengfei
Chen, Bijun
Sun, Zilin
Source
International Journal of Endocrinology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background and Aims.
We here assess the effects of PSCs on β-cell function and apoptosis in vivo and in vitro.
Materials and Methods.
PSCs were transplanted into Wistar and Goto-Kakizaki (GK) rats.
Sixteen weeks after transplantation, β-cell function, apoptosis, and islet fibrosis were assessed.
In vitro the effects of PSCs conditioned medium (PSCs-CM) and/or high concentration of glucose on INS-1 cell function was assessed by measuring insulin secretion, INS-1 cell survival, apoptosis, and endoplasmic reticulum stress (ER stress) associated CHOP expression.
Results.
PSCs transplantation exacerbated the impaired β-cell function in GK rats, but had no significant effects in Wistar rats.
In vitro, PSCs-CM caused impaired INS-1 cell viability and insulin secretion and increased apoptosis, which were more pronounced in the presence of high glucose.
Conclusion.
Our study demonstrates that PSCs induce β-cell failure in vitro and in vivo.
American Psychological Association (APA)
Zha, Min& Xu, Wei& Zhai, Qing& Li, Fengfei& Chen, Bijun& Sun, Zilin. 2014. High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function. International Journal of Endocrinology،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1036421
Modern Language Association (MLA)
Zha, Min…[et al.]. High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function. International Journal of Endocrinology No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1036421
American Medical Association (AMA)
Zha, Min& Xu, Wei& Zhai, Qing& Li, Fengfei& Chen, Bijun& Sun, Zilin. High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function. International Journal of Endocrinology. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1036421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036421