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Alterations in the Corneal Nerve and StemProgenitor Cells in Diabetes: Preventive Effects of Insulin-Like Growth Factor-1 Treatment
Joint Authors
Suzuki, Noboru
Ueno, Hiroki
Hattori, Takaaki
Kumagai, Yuta
Ueno, Satoki
Takagi, Hitoshi
Source
International Journal of Endocrinology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study aimed to investigate whether corneal nerve and corneal stem/progenitor cells are altered in insulin-like growth factor-I (IGF-I-) treated individuals with diabetes.
A group consisting of db/db mice with type 2 diabetes mellitus (DM) and a wild-type group were assessed by neural and corneal stem/progenitor cell markers immunostaining and real-time PCR.
Moreover, the expression of corneal nerve and stem/progenitor cell markers was examined in IGF-1-treated diabetic mice.
Compared with a normal cornea, swelling and stratification of the corneal epithelium were noted in db/db mice.
Beta-III tubulin immunostaining revealed that the corneal subbasal plexuses in diabetic mice were thinner with fewer branches.
mRNA expression levels of Hes1, Keratin15, and p75 (corneal stem/progenitor cell markers) and the intensity and number of positive cells of Hes1 and Keratin19 immunostaining diminished in the diabetic corneas.
Compared with the subbasal nerve density in the normal group, a decrease in the diabetic group was observed, whereas the corneal subbasal nerve density increased in IGF-1-treated diabetic group.
The decreased expression of Hes1 and Keratin19 was prevented in IGF-1-treated diabetic group.
Our data suggest that corneal nerve and stem/progenitor cells are altered in type 2 DM, and IGF-I treatment is capable of protecting against corneal damage in diabetes.
American Psychological Association (APA)
Ueno, Hiroki& Hattori, Takaaki& Kumagai, Yuta& Suzuki, Noboru& Ueno, Satoki& Takagi, Hitoshi. 2014. Alterations in the Corneal Nerve and StemProgenitor Cells in Diabetes: Preventive Effects of Insulin-Like Growth Factor-1 Treatment. International Journal of Endocrinology،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1036460
Modern Language Association (MLA)
Ueno, Hiroki…[et al.]. Alterations in the Corneal Nerve and StemProgenitor Cells in Diabetes: Preventive Effects of Insulin-Like Growth Factor-1 Treatment. International Journal of Endocrinology No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1036460
American Medical Association (AMA)
Ueno, Hiroki& Hattori, Takaaki& Kumagai, Yuta& Suzuki, Noboru& Ueno, Satoki& Takagi, Hitoshi. Alterations in the Corneal Nerve and StemProgenitor Cells in Diabetes: Preventive Effects of Insulin-Like Growth Factor-1 Treatment. International Journal of Endocrinology. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1036460
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036460