Electron Transfer of Myoglobin Immobilized in Au Electrodes Modified with a RAFT PMMA-Block-PDMAEMA Polymer
Joint Authors
Toledo, Carla N.
Florenzano, Fábio H.
Schneedorf, José M.
Source
International Journal of Electrochemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Myoglobin was immobilized with poly(methyl methacrylate)-block-poly[(2-dimethylamino)ethyl methacrylate]PMMA-block-PDMAEMA polymer synthesized by reversible addition-fragmentation chain transfer technique (RAFT).
Cyclic voltammograms gave direct and slow quasireversible heterogeneous electron transfer kinetics between Mb-PMMA-block-PDMAEMA modified electrode and the redox center of the protein.
The values for electron rate constant (Ks) and transfer coefficient (α) were 0.055±0.01·s−1 and 0.81±0.08, respectively.
The reduction potential determined as a function of temperature (293–328 K) revealed a value of reaction center entropy of ΔS0 of 351.3±0.0002 J·mol−1·K−1 and enthalpy change of -76.8±0.1 kJ·mol−1, suggesting solvent effects and charge ionization atmosphere involved in the reaction parallel to hydrophobic interactions with the copolymer.
The immobilized protein also exhibits an electrocatalytical response to reduction of hydrogen peroxide, with an apparent Km of 114.7±58.7 μM.
The overall results substantiate the design and use of RAFT polymers towards the development of third-generation biosensors.
American Psychological Association (APA)
Toledo, Carla N.& Florenzano, Fábio H.& Schneedorf, José M.. 2014. Electron Transfer of Myoglobin Immobilized in Au Electrodes Modified with a RAFT PMMA-Block-PDMAEMA Polymer. International Journal of Electrochemistry،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1036646
Modern Language Association (MLA)
Toledo, Carla N.…[et al.]. Electron Transfer of Myoglobin Immobilized in Au Electrodes Modified with a RAFT PMMA-Block-PDMAEMA Polymer. International Journal of Electrochemistry No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1036646
American Medical Association (AMA)
Toledo, Carla N.& Florenzano, Fábio H.& Schneedorf, José M.. Electron Transfer of Myoglobin Immobilized in Au Electrodes Modified with a RAFT PMMA-Block-PDMAEMA Polymer. International Journal of Electrochemistry. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1036646
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036646