![](/images/graphics-bg.png)
Macrophage Derived Platelet Activating Factor Implicated in the Resolution Phase of Gouty Inflammation
Author
Source
International Journal of Inflammation
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Human blood derived in vitro differentiated monocytes or macrophages are a population of cells which have been investigated over the years to determine the role these cells play in the resolution phase of gout.
Macrophages are able to phagocytose monosodium urate monohydrate (MSU) crystals without releasing inflammatory factors.
This study analysed macrophage platelet activating factor secretion and its possible role in the pathway of gout resolution.
Analysis of sunatants from in vitro differentiated macrophages stimulated with MSU crystals revealed the secretion of platelet activating factor (PAF) 1.54 ± 0.10 mean ± SEM; ng/mL per 106 cells.
This secretion was absent in immature monocytes treated similarly.
When these monocytes were pretreated with recombinant human PAF-acetylhydrolase (rhuPAF-AH) and MSU crystals resulted in TNFα suppression.
Addition of WEB2086, a platelet activating factor (PAF) antagonist, to differentiated macrophages with MSU crystals unmasked TNFα secretion 0.7 ± 0.06 mean ± SEM; ng/mL per 106 cells.
This study identifies a role for PAF and the PAF receptor antagonist in the pathway by which macrophages ingest MSU crystals and resolve the concomitant inflammation.
American Psychological Association (APA)
Yagnik, Darshna. 2014. Macrophage Derived Platelet Activating Factor Implicated in the Resolution Phase of Gouty Inflammation. International Journal of Inflammation،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1036725
Modern Language Association (MLA)
Yagnik, Darshna. Macrophage Derived Platelet Activating Factor Implicated in the Resolution Phase of Gouty Inflammation. International Journal of Inflammation No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1036725
American Medical Association (AMA)
Yagnik, Darshna. Macrophage Derived Platelet Activating Factor Implicated in the Resolution Phase of Gouty Inflammation. International Journal of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1036725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036725