Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture

Joint Authors

Zhang, Cui-Ping
Li, Shao-Dan
Chen, Yan
Jiang, Yan-Ming
Chen, Peng
Wang, Chang-Zhen
Kang, Hong-Xiang
Shen, Ben-Jian
Liang, Jie
Fu, Xiaobing

Source

International Journal of Photoenergy

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-07-21

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Chemistry

Abstract EN

Low intensity laser irradiation (LILI) was found to promote the regeneration of skeletal muscle in vivo but the cellular mechanisms are not fully understood.

Myoblasts, normally quiescent and inactivated in adult skeletal muscle, are a type of myogenic progenitor cells and considered as the major candidates responsible for muscle regeneration.

The aim of the present study was to study the effect of LILI on the growth potential and cell-cycle progression of the cultured myoblasts.

Primary myoblasts isolated from rat hind legs were cultured in nutrient-deficient medium for 36 hours and then irradiated by helium-neon laser at a certain energy density.

Immunohistochemical and flow cytometric analysis revealed that laser irradiation could increase the expression of cellular proliferation marker and the amount of cell subpopulations in the proliferative phase as compared with the nonirradiated control group.

Meanwhile, the expressions of cell-cycle regulatory proteins in the laser-treated myoblasts were markedly upregulated as compared to the unirradiated cells, indicating that LILI could promote the reentry of quiescent myoblasts into the cell division cycle.

These results suggest that LILI at certain fluences could promote their proliferation, thus contributing to the skeletal muscle regeneration following trauma and myopathic diseases.

American Psychological Association (APA)

Zhang, Cui-Ping& Li, Shao-Dan& Chen, Yan& Jiang, Yan-Ming& Chen, Peng& Wang, Chang-Zhen…[et al.]. 2014. Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture. International Journal of Photoenergy،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1036799

Modern Language Association (MLA)

Zhang, Cui-Ping…[et al.]. Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture. International Journal of Photoenergy No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1036799

American Medical Association (AMA)

Zhang, Cui-Ping& Li, Shao-Dan& Chen, Yan& Jiang, Yan-Ming& Chen, Peng& Wang, Chang-Zhen…[et al.]. Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture. International Journal of Photoenergy. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1036799

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1036799