Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B
Joint Authors
Hwang, Ok-Jin
Lim, Soo-Hyun
Han, Yun-Jeong
Shin, Ah-Young
Kim, Do-Soon
Kim, Jeong-Il
Source
International Journal of Photoenergy
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Phytochromes are dimeric pigment proteins with reversible photochromism between red and far-red light-absorbing forms.
They are photoreceptors that regulate various aspects of plant growth and development and have been used for biotechnological applications to improve agricultural performance of crops.
Miscanthus species have been suggested as one of the most promising energy crops.
In this paper, Arabidopsis phytochrome B (PHYB) gene was introduced into Miscanthus sinensis using Agrobacterium-mediated transformation method that we developed recently, with the herbicide resistance gene (BAR) as a selection marker.
After putative transgenic plants were selected using the herbicide resistance assay, genomic integration of the transgene was confirmed by genomic PCR and Southern blot analysis, and transgene expression was validated by Northern blot analysis.
Compared to nontransformed control plants, transgenic plants overexpressing PHYB showed phenotypes with increased phytochrome B function, which includes increased chlorophyll content, decreased plant height, and delayed flowering.
Therefore, these results suggest that Arabidopsis phytochrome B is functional in M.
sinensis and provide a method to develop Miscanthus varieties with enhanced agricultural performance using phytochromes.
American Psychological Association (APA)
Hwang, Ok-Jin& Lim, Soo-Hyun& Han, Yun-Jeong& Shin, Ah-Young& Kim, Do-Soon& Kim, Jeong-Il. 2014. Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B. International Journal of Photoenergy،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1036980
Modern Language Association (MLA)
Hwang, Ok-Jin…[et al.]. Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B. International Journal of Photoenergy No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1036980
American Medical Association (AMA)
Hwang, Ok-Jin& Lim, Soo-Hyun& Han, Yun-Jeong& Shin, Ah-Young& Kim, Do-Soon& Kim, Jeong-Il. Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B. International Journal of Photoenergy. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1036980
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036980