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Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage
Joint Authors
Jiang, Yuan
Hua, Heyu
Rao, Xiancai
Leung, Albert Wingnang
Xu, Chuanshan
Source
International Journal of Photoenergy
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Aim.
To investigate the effect of photodynamic action of LED-activated curcumin on cell viability, membrane permeability, and intracellular reactive oxygen species of Staphylococcus aureus.
Methods.
Staphylococcus aureus was incubated with the different concentrations of curcumin for 60 min and then irradiated by blue light with the wavelength of 470 nm and with light dose of 3 J/cm2.
The colony forming unit assay was used to investigate photocytotoxicity of curcumin on Staphylococcus aureus, confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) for assaying membrane permeability, FCM analysis with DCFH-DA staining for measuring the intracellular ROS level, and transmission electron microscopy (TEM) for observing morphology and structure.
Results.
Blue light-activated curcumin significantly killed Staphylococcus aureus in a curcumin dose-dependent manner.
TEM observed remarkable structural damages in S.
aureus after light-activated curcumin.
More red fluorescence of PI dye was found in S.
aureus treated by blue light-activated curcumin than in those of the controlled bacterial cells.
Intracellular ROS increase was observed after light-activated curcumin.
Conclusion.
Blue light-activated curcumin markedly damaged membrane permeability, resulting in cell death of Staphylococcus aureus and highlighted that intracellular ROS increase might be an important event in photodynamic killing of Staphylococcus aureus in the presence of curcumin.
American Psychological Association (APA)
Jiang, Yuan& Leung, Albert Wingnang& Hua, Heyu& Rao, Xiancai& Xu, Chuanshan. 2014. Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage. International Journal of Photoenergy،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1037264
Modern Language Association (MLA)
Jiang, Yuan…[et al.]. Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage. International Journal of Photoenergy No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1037264
American Medical Association (AMA)
Jiang, Yuan& Leung, Albert Wingnang& Hua, Heyu& Rao, Xiancai& Xu, Chuanshan. Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage. International Journal of Photoenergy. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1037264
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1037264