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Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells
Joint Authors
Mi, Xian-qiang
Zhong, Fu-qing
Li, Yang
Source
International Journal of Photoenergy
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background and Objective.
ATP-sensitive potassium (KATP) channel couples cell metabolism to excitability.
To explore role of KATP channels in cellular photobiomodulation, we designed experiment to study effect of low intensity 808 nm laser irradiation on the activity of membrane KATP channel.
Study Design/Materials and Methods.
Plasmids encoding Kir6.2 was constructed and heterologously expressed in cultured mammalian HEK-293 cells.
The patch-clamp and data acquisition systems were used to record KATP channel current before and after irradiation.
A laser beam of Ga-As 808 nm at 5 mW/cm2 was used in experiments.
A one-way ANOVA test followed by a post hoc Student-Newman-Keuls test was used to assess the statistical differences between data groups.
Results.
Obvious openings of KATP channels of Kir6.2-transfected HEK-293 cells and excised patches were recorded during and after low intensity 808 nm laser irradiation.
Compared with the channels that did not undergo irradiation, open probability, current amplitude, and dwell time of KATP channels after irradiation improved.
Conclusions.
Low intensity 808 nm laser irradiation may activate membrane KATP channels of Kir6.2-transfected HEK-293 cells and in excised patches.
American Psychological Association (APA)
Zhong, Fu-qing& Li, Yang& Mi, Xian-qiang. 2014. Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells. International Journal of Photoenergy،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1037402
Modern Language Association (MLA)
Zhong, Fu-qing…[et al.]. Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells. International Journal of Photoenergy No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1037402
American Medical Association (AMA)
Zhong, Fu-qing& Li, Yang& Mi, Xian-qiang. Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells. International Journal of Photoenergy. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1037402
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1037402