Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF6A Cells by 670 nm Light-Emitting Diode Irradiation

Abstract EN

Objective.

The goal of this study was to investigate the therapeutic efficacy of 670 nm light-emitting diode (LED) irradiation on the diabetic retinopathy (DR) using hypoxic rhesus monkey choroid-retinal (RF/6A) cells as the model system.

Background Data.

Treatment with light in the spectrum from red to near-infrared region has beneficial effect on tissue injury and 670 nm LED is currently under clinical investigation for retinoprotective therapy.

Methods.

Studies were conducted in the cultured cells under hypoxia treated by cobalt chloride (CoCl2).

After irradiation by 670 nm LED with different power densities, cell viability, cytochrome C oxidase activity, and ATP concentration were measured.

Results.

The irradiation of 670 nm LED significantly improved cell viability, cytochrome C oxidase activity, and ATP concentration in the hypoxia RF/6A cells.

Conclusion.

670 nm LED irradiation could recover the hypoxia damage caused by CoCl2.

Photobiomodulation of 670 nm LED plays a potential role for the treatment of diabetic retinopathy.