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Enhancement of Fluorescent Labeling via a Composited Thin Film
Joint Authors
Suyama, Taikei
Ji, Xiaowei
Zhang, Yaoju
Source
International Journal of Polymer Science
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Fluorescent labeling is the prevailing imaging technique in cell biological research.
When statistical investigations on a large number of cells are involved, experimental study is required for both low magnification to get a reliable statistical population and high contrast to achieve accurate diagnosis on the nature of the cells’ perturbation.
As microscope objectives of low magnification generally yield low collection efficiency, such studies are limited by the fluorescence signal weakness.
To overcome this technological insufficiency, Le Moal et al.
proposed a method based on metal-coated substrates that enhanced the fluorescence process and improved collection efficiency in fluorescence microscope observation and that could be directly used with a common microscope setup.
In this paper, we use an Ag-Si3N4-Ag multilayer film coated on the substrate and numerically analyse the optical behavior of a fluorophore which was placed above the composited film coated on the substrate.
The results shows that by using an Ag-Si3N4-Ag composited film the fluorescence imaging can be enhanced remarkably.
American Psychological Association (APA)
Suyama, Taikei& Ji, Xiaowei& Zhang, Yaoju. 2014. Enhancement of Fluorescent Labeling via a Composited Thin Film. International Journal of Polymer Science،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1037590
Modern Language Association (MLA)
Suyama, Taikei…[et al.]. Enhancement of Fluorescent Labeling via a Composited Thin Film. International Journal of Polymer Science No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1037590
American Medical Association (AMA)
Suyama, Taikei& Ji, Xiaowei& Zhang, Yaoju. Enhancement of Fluorescent Labeling via a Composited Thin Film. International Journal of Polymer Science. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1037590
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1037590