Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System

Joint Authors

Ma, Jin Yeul
Lee, Kwang Jin
Oh, You-Chang
Song, Na-Young
Cho, Won-Kyung

Source

Journal of Analytical Methods in Chemistry

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-10-15

Country of Publication

Egypt

No. of Pages

15

Main Subjects

Chemistry

Abstract EN

The Acer tegmentosum (3 kg) was extracted using hot water, and the freeze-dried extract powder was partitioned successively using dichloromethane (DCM), ethyl acetate (EA), butyl alcohol (n-BuOH), and water.

From the EA extract fraction (1.24 g), five phenolic compounds were isolated by the silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography.

Based on spectroscopic methods such as 1H-NMR, 13C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5).

Moreover, a rapid on-line screening HPLC-ABTS+ system for individual bioactivity of the EA-soluble fraction (five phenolic compounds) was developed.

The results indicated that compounds 1 and 2 were first isolated from the A.

tegmentosum.

The anti-inflammatory activities and on-line screening HPLC-ABTS+ assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A.

tegmentosum.

American Psychological Association (APA)

Lee, Kwang Jin& Song, Na-Young& Oh, You-Chang& Cho, Won-Kyung& Ma, Jin Yeul. 2014. Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1039817

Modern Language Association (MLA)

Lee, Kwang Jin…[et al.]. Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1039817

American Medical Association (AMA)

Lee, Kwang Jin& Song, Na-Young& Oh, You-Chang& Cho, Won-Kyung& Ma, Jin Yeul. Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1039817

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1039817