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Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System
Joint Authors
Ma, Jin Yeul
Lee, Kwang Jin
Oh, You-Chang
Song, Na-Young
Cho, Won-Kyung
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-15
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The Acer tegmentosum (3 kg) was extracted using hot water, and the freeze-dried extract powder was partitioned successively using dichloromethane (DCM), ethyl acetate (EA), butyl alcohol (n-BuOH), and water.
From the EA extract fraction (1.24 g), five phenolic compounds were isolated by the silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography.
Based on spectroscopic methods such as 1H-NMR, 13C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5).
Moreover, a rapid on-line screening HPLC-ABTS+ system for individual bioactivity of the EA-soluble fraction (five phenolic compounds) was developed.
The results indicated that compounds 1 and 2 were first isolated from the A.
tegmentosum.
The anti-inflammatory activities and on-line screening HPLC-ABTS+ assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of A.
tegmentosum.
American Psychological Association (APA)
Lee, Kwang Jin& Song, Na-Young& Oh, You-Chang& Cho, Won-Kyung& Ma, Jin Yeul. 2014. Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1039817
Modern Language Association (MLA)
Lee, Kwang Jin…[et al.]. Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1039817
American Medical Association (AMA)
Lee, Kwang Jin& Song, Na-Young& Oh, You-Chang& Cho, Won-Kyung& Ma, Jin Yeul. Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1039817
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1039817